Isolation of new Pseudomonas tolaasii bacteriophages and genomic investigation of the lytic phage BF7

FEMS Microbiology Letters
Enikő Sajben-NagyLászló Manczinger

Abstract

Sixteen lytic bacteriophages that infect Pseudomonas tolaasii LMG 2342(T) were isolated from smashed sporocarps of oyster mushroom (Pleurotus ostreatus) showing necrotic symptoms. On the basis of the host range investigation of the phages, they have wide infection abilities against the genus Pseudomonas, mainly in the case of phages Bf3, Bf7, Bf10, and Bf15. Molecular investigations have revealed that they all have dsDNA genomes about 40 kbp in size. Identical restriction patterns resulting from restriction enzyme analysis suggest that the isolates probably belong to the same phage species. However, there was a difference between these phage isolates in their infecting abilities. Phage isolate Bf7 was investigated and characterized more deeply. Morphological characterization of Bf7 by transmission electron microscopy (TEM) has shown that it has a short, noncontractile tail, an icosahedral phage head, and the size is about 60 nm in diameter, suggesting that it belongs to the Podoviridae family. Complete genome sequence analysis of the Bf7 phage isolate revealed a 40 058 bp genome, 58.4% G+C content, 46 open reading frames encoding different proteins showing homology to proteins of the bacteriophage Caulobacter crescentus φCd1 fr...Continue Reading

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Citations

Apr 7, 2017·Intervirology·Ira Aryani WirjonYahya Mat Arip
Jul 8, 2018·Applied and Environmental Microbiology·Chikage TanakaTakahiro Toba
Sep 29, 2019·Plant Disease·Ebrahim OsdaghiCarolee T Bull
Mar 6, 2021·Frontiers in Microbiology·Felipe MolinaJosé Emilio Rebollo

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