PMID: 7527283May 1, 1994Paper

Isolation of non-LTR retrotransposon reverse transcriptase-like sequences from phlebotomine sandflies

Insect Molecular Biology
D R BoothD F Smith

Abstract

Reverse transcriptase-like sequences (RTs) with amino acid motifs characteristic of non-LTR retrotransposons have been isolated from several medically important phlebotomine sandfly species. These sequences were amplified using the polymerase chain reaction (PCR) with primers based on conserved amino acid motifs present in previously described insect RTs. A further set of RTs were amplified using primers based on the conserved regions identified in phlebotomine RTs. The average similarity of the phlebotomine RTs to the Drosophila I, F and R1Dm elements was 28-29% between the closest primers used. Phlebotomine RTs were 31-62% similar to each other, the most dissimilar sequences coming from the same species. Several amino acid residues were invariant in the ten phlebotomine RTs, including motif Q(F/Y)GF, conserved in other non-LTR retrotransposons, but not in retrovirus or LTR retrotransposon RTs. The remarkable conservation of this distinctive domain of non-LTR retrotransposon RTs suggests it has a vital and possibly unique role in the mode of reverse transcription of this class of transposon.

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Citations

Jul 10, 2010·International Journal of Environmental Research and Public Health·Hirotomo KatoYoshihisa Hashiguchi
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jérôme Depaquit
Jul 11, 2006·Journal of Molecular Evolution·Alicia E NovakAngeles B Ribera
Oct 13, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rehab F AbdelhamidHiroshi Hori
May 21, 2014·ZooKeys·Frederico F SallesMichel Sartori

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