Isolation of nucleic acid binding proteins: an approach for isolation of cell surface, nucleic acid binding proteins

Annals of the New York Academy of Sciences
Boris P ChelobanovValentin V Vlassov

Abstract

An approach for isolation of cell surface, nucleic acid binding proteins is described. This approach relies on affinity modification of the proteins of living cells with reactive oligonucleotides bearing a haptenic group. Covalently modified proteins were isolated by hapten-specific affinity chromatography with subsequent SDS-PAGE. Isolated 68-kDa proteins responsible for the binding of oligonucleotides were MS/MS sequenced and identified as keratin K1, keratin K10, keratin K2e, and albumin.

References

Jan 1, 1992·Antisense Research and Development·V G BudkerV V Vlassov
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L A YakubovV V Vlassov
Sep 21, 2001·Nucleosides, Nucleotides & Nucleic Acids·P LaktionovV Vlassov
Nov 6, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·M StrounP Anker
Jul 15, 2004·Annals of the New York Academy of Sciences·Pavel P LaktionovValentin V Vlassov

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Citations

Jun 16, 2010·Breast Cancer Research and Treatment·Anne M FrielLorraine O'Driscoll
Jul 11, 2006·Biochemistry. Biokhimii︠a︡·B P ChelobanovV V Vlasov
Sep 10, 2008·Yi chuan = Hereditas·Ying Hu, Hong Ni
Apr 29, 2008·Journal of Molecular Recognition : JMR·Irina O PetrusevaOlga I Lavrik
Dec 2, 2006·Biochimica Et Biophysica Acta·M Fleischhacker, B Schmidt
Feb 27, 2010·Cellular & Molecular Biology Letters·Marketa UrbanovaJan Betka
Aug 29, 2006·Bioorganic & Medicinal Chemistry Letters·Yuliya V GerasimovaTatyana S Godovikova

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