PMID: 52572Aug 1, 1975Paper

Isolation of parietal cells from guinea-pig gastric mucosa and the immunological characterization of their antigenic structure

Gut
D P JewellJ P Wright

Abstract

A method is described for the isolation of parietal cells from the gastric mucosa of the guinea pig by enzymatic digestion with collagenase. A suspension was obtained that contained 70-80% parietal cells. About 80% of the cells were viable immediately after incubation, but viability dropped sharply after one hour. Parietal cells were identified by their morphology on light and electron microscopy, by their uptake of neutral red, by immunofluorescent staining and by carbonic anhydrase activity. Antibodies to four distinct parietal-cell antigens were obtained from rabbits immunized with the isolated parietal cells or fractions thereof. These antibodies were directed against the microsomal fraction of the parietal-cell cytoplasm, the plasma and nuclear membranes, the soluble proteins, and Castle's intrinsic factor. The antibody against the microsomal fraction, though reacting in the same way as the antibody to parietal cell canaliculi found in the serum of patients with pernicious anaemia, showed greater species specificity.

References

Jul 1, 1957·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M NACHLASA M SELIGMAN
Feb 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·A I WALDER, J B LUNSETH
Nov 1, 1963·Analytical Biochemistry·R KARLER, D M WOODBURY
Mar 11, 1965·The New England Journal of Medicine·J M FISHER, K B TAYLOR
Mar 1, 1951·The Australian Journal of Experimental Biology and Medical Science·M DONNELLEY
Apr 1, 1950·The Biochemical Journal·N M BRADFORD, R E DAVIES
Sep 20, 1938·The Journal of General Physiology·M L Anson

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Citations

May 1, 1981·Agents and Actions·M Albinus, K F Sewing
Apr 4, 1978·Biochimica Et Biophysica Acta·S Batzri, J D Gardner
Jan 1, 1980·International Urology and Nephrology·S I RyabovE V Bakhidze
Jan 1, 1989·Microbiology and Immunology·K Masuda, T Kawata
Oct 3, 2019·Diseases of Aquatic Organisms·Jamie Bojko, Mykola Ovcharenko
Jan 3, 1978·Biochimica Et Biophysica Acta·J Brown, N D Gallagher

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