Isolation of Peripheral Blood CD8 T Cells Specific to Porcine Reproductive and Respiratory Syndrome Virus Utilizing Porcine CD137 Activation Marker

Viral Immunology
Sakthivel SubramaniamXiang-Jin Meng

Abstract

CD137 is a costimulatory molecule transiently expressed on activated T cells after mitogen or antigen stimulation that can be exploited for isolating antigen-specific T cells as reported in mouse models. By utilizing an antiporcine CD137 monoclonal antibody (mAb, clone 3B9) developed in our laboratory, we isolated virus-specific CD8β T cells from peripheral blood of pigs experimentally infected with different porcine reproductive and respiratory syndrome virus (PRRSV) strains. Similar to mouse, porcine CD8β T cells also express CD137 transiently upon Concavalin A stimulation while the unstimulated cells did not. Most frequently, virus-specific CD8β T cells were isolated at low levels from peripheral blood of pigs experimentally infected with PRRSV strains VR2385, NADC20, and MN184B at 49 and 63 days postinfection. The results suggest that porcine CD137-specific mAb is a useful tool for isolating virus-specific CD8 T cells from peripheral blood and tissues of pigs after in vitro stimulation with viral antigen.

References

Nov 25, 1998·Seminars in Immunology·D S Vinay, B S Kwon
Aug 27, 2005·Journal of the American Veterinary Medical Association·Eric J NeumannJeffrey J Zimmerman
Jun 19, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matthias WölflPhilip D Greenberg
Jul 8, 2008·Developmental and Comparative Immunology·Wilhelm GernerArmin Saalmüller
Aug 5, 2008·International Journal of Hematology·Kazue WatanabeYoshiki Akatsuka
Mar 5, 2013·Veterinary Immunology and Immunopathology·Xinxin ZhaoXun Suo
Jun 22, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Petra Bacher, Alexander Scheffold

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Methods Mentioned

BETA
density gradient centrifugation
fluorescence-activated cell sorting
Flow cytometry
ELISA
FACS

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