Isolation of Peroxisomes from Yeast

Cold Spring Harbor Protocols
Jana CramerRalf Erdmann

Abstract

Peroxisomes are multifunctional, dynamic organelles present in nearly all eukaryotic cells. Determining their structural and functional characteristics often requires obtaining isolated and purified peroxisomes via subcellular fractionation. Subcellular fractionation techniques are generally based on a three-step procedure: preparation of a cell-free homogenate (postnuclear supernatant), generation of an organellar pellet by differential centrifugation, and density gradient centrifugation. Here we introduce methods for small-scale isolation of peroxisomes from yeast cells using different gradient media as well as large-scale purification using a two-step gradient centrifugation.

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Sep 4, 2015·Cold Spring Harbor Protocols·Jana CramerRalf Erdmann
Sep 4, 2015·Cold Spring Harbor Protocols·Jana CramerRalf Erdmann

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Citations

May 30, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Justyna P WróblewskaIda J van der Klei
Aug 16, 2015·The Journal of Biological Chemistry·Daniel EffelsbergRalf Erdmann
Sep 12, 2015·The Journal of Biological Chemistry·Stefanie HagenRalf Erdmann
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Katharina ReglinskiRalf Erdmann
Nov 13, 2020·Journal of Cell Science·Shiran Gabay-MaskitEinat Zalckvar

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