Isolation of phages for phage therapy: a comparison of spot tests and efficiency of plating analyses for determination of host range and efficacy

PloS One
Mohammadali Khan Mirzaei, Anders S Nilsson

Abstract

Phage therapy, treating bacterial infections with bacteriophages, could be a future alternative to antibiotic treatment of bacterial infections. There are, however, several problems to be solved, mainly associated to the biology of phages, the interaction between phages and their bacterial hosts, but also to the vast variation of pathogenic bacteria which implies that large numbers of different phages are going to be needed. All of these phages must under present regulation of medical products undergo extensive clinical testing before they can be applied. It will consequently be of great economic importance that effective and versatile phages are selected and collected into phage libraries, i.e., the selection must be carried out in a way that it results in highly virulent phages with broad host ranges. We have isolated phages using the Escherichia coli reference (ECOR) collection and compared two methods, spot testing and efficiency of plating (EOP), which are frequently used to identify phages suitable for phage therapy. The analyses of the differences between the two methods show that spot tests often overestimate both the overall virulence and the host range and that the results are not correlated to the results of EOP assa...Continue Reading

References

Mar 1, 1991·Journal of General Microbiology·P BeltranR K Selander
Jan 1, 1971·Advances in Genetics·L E Bertani, G Bertani
Feb 1, 1984·Journal of Bacteriology·H Ochman, R K Selander
Jun 1, 1993·Journal of General Microbiology·E F BoydR K Selander
May 16, 1998·Current Biology : CB·E S Weinberg
Sep 2, 2003·Molecular Biology and Evolution·Anders S NilssonElisabeth Haggård-Ljungquist
Dec 9, 2003·Applied and Environmental Microbiology·Stephen T AbedonCameron Thomas
Mar 3, 2005·The Science of the Total Environment·S WitheyT Stephenson
Dec 11, 2008·Methods in Molecular Biology·Elizabeth Kutter
Dec 11, 2008·Methods in Molecular Biology·Paul Hyman, Stephen T Abedon
Dec 18, 2008·FEMS Microbiology Letters·Kenshi MatsushitaShigenobu Matsuzaki
Jun 13, 2009·Environmental Microbiology·Andrew M KropinskiRob Lavigne
Jan 1, 2009·Current Pharmaceutical Biotechnology·Jason J Gill, Paul Hyman
Jan 1, 2009·Current Pharmaceutical Biotechnology·Stephen T Abedon, Cameron Thomas-Abedon
Jan 1, 2009·Current Pharmaceutical Biotechnology·Elizabeth KutterStephen T Abedon
Apr 3, 2010·Advances in Applied Microbiology·Paul Hyman, Stephen T Abedon
Nov 11, 2010·Pharmaceutical Research·Jean-Paul PirnayRevaz Adamia
Mar 3, 2011·Journal of Applied Microbiology·S ViazisF Diez-Gonzalez
Jun 21, 2011·Bacteriophage·Stephen T Abedon
Feb 16, 2012·Bacteriophage·Stephen T AbedonElizabeth Martin Kutter
Aug 14, 2012·Applied and Environmental Microbiology·Hans Petter KleppenSung-Sik Yoon
Nov 26, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Harald Brüssow
Nov 26, 2013·Applied and Environmental Microbiology·Minsik KimSangryeol Ryu
Feb 4, 2014·Applied and Environmental Microbiology·Rebekah A FramptonPeter C Fineran
Feb 7, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Gilbert VerbekenIsabelle Huys
Mar 13, 2014·Bacteriophage·Eric C Keen
Apr 1, 2014·Upsala Journal of Medical Sciences·Anders S Nilsson

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Citations

Jul 9, 2016·The Journal of Antimicrobial Chemotherapy·Nicolas DufourUNKNOWN ColoColi group
Aug 19, 2016·Frontiers in Microbiology·Callum J CooperAnders S Nilsson
Aug 30, 2016·Frontiers in Microbiology·Beata Weber-DąbrowskaAndrzej Górski
Sep 24, 2016·Frontiers in Microbiology·Alexa RossPaul Hyman
Dec 16, 2016·FEMS Microbiology Letters·Andrei Sorin BolocanColin Hill
May 29, 2016·Environmental Microbiology·Karin HolmfeldtMatthew B Sullivan
Mar 23, 2018·Diseases of Aquatic Organisms·Truong Dinh HoaiToshihiro Nakai
Jul 26, 2018·Veterinary Research Communications·Diego F Varela-OrtizAbner J Gutiérrez-Chávez
Nov 15, 2019·Upsala Journal of Medical Sciences·Anders S Nilsson
Apr 7, 2018·Journal of Food Protection·Pushpinder Kaur LittDivya Jaroni
Sep 10, 2020·Cellular & Molecular Immunology·Sara FedericiEran Elinav
Jan 9, 2019·Frontiers in Microbiology·Chaojie YangHongbin Song
Jun 8, 2018·FEMS Microbiology Ecology·Clara Torres-BarcelóMichael E Hochberg
Jul 31, 2018·The Journal of Antimicrobial Chemotherapy·Tristan FerryUNKNOWN Lyon Bone and Joint Infection Study Group
Dec 12, 2018·Microbiology Resource Announcements·Naveen ChaudharyNeelam Taneja
Jun 21, 2019·Viruses·Edel StoneOlivia McAuliffe
May 14, 2020·Journal of Environmental Health Science & Engineering·Cheonghoon LeeMi-Kyung Park
Dec 22, 2017·Letters in Applied Microbiology·T PimchanA S Nilsson
Sep 8, 2017·African Journal of Laboratory Medicine·Joseph M Ochieng' OduorAtunga Nyachieo
Oct 26, 2018·PloS One·Prasanth ManoharNachimuthu Ramesh
Feb 14, 2019·Folia Microbiologica·Esra Acar SoykutSami Fattouch

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