Isolation of pig bone marrow mesenchymal stem cells suitable for one-step procedures in chondrogenic regeneration

Journal of Tissue Engineering and Regenerative Medicine
A Peterbauer-ScherbS Wolbank

Abstract

Large animals such as pigs are good models for skeletal tissue engineering, since they provide physical forces similar to those of humans. Porcine bone marrow mesenchymal stem cells (BMSCs) have shown regenerative capacity similar to those of human BMSCs and can therefore be preclinically applied in settings corresponding to autologous transplantation in patients. Aiming at a one-step procedure for cartilage regeneration with autologous BMSCs, three straightforward isolation methods for BMSCs of Göttingen minipigs were compared. For this purpose, the BMSC fraction was enriched by red blood cell (RBC) lysis, dextran sedimentation or density gradient centrifugation. Isolated BMSCs were evaluated with regard to cell yield, proliferation capacity, phenotype and ability to differentiate to the chondrogenic lineage. Highest cell yields determined at the time of subcultivation were obtained using RBC lysis. In comparison, dextran sedimentation was less efficient but superior to density gradient centrifugation, which yielded significantly lower cell numbers than RBC lysis. The evaluated isolation methods resulted in cultures with equal proliferative capacity, with constant population doubling times of 50-55 h for at least 100 days (app...Continue Reading

References

Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Oct 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B M ThomsonN Loveridge
Mar 21, 2002·Cell and Tissue Research·Jochen RingeMichael Sittinger
Jun 14, 2002·The Journal of Thoracic and Cardiovascular Surgery·Shinji TomitaRen-Ke Li
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Jingbo LiuJianyi Zhang
May 10, 2005·Journal of Cellular Physiology·Victor VacantiTechung Lee
Jul 1, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Xiaobing FuZhiyong Sheng
Nov 24, 2006·Advances in Experimental Medicine and Biology·Xuenong ZouCody Bünger
Feb 27, 2008·Journal of Cellular and Molecular Medicine·R J W HoogendoornM N Helder
Nov 22, 2008·The Journal of Pathology·A I Caplan
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Gyu Jin RhoS S Balasubramanian
May 19, 2009·Clinical Orthopaedics and Related Research·Sandro GianniniBrunella Grigolo

❮ Previous
Next ❯

Citations

Dec 29, 2011·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·K GavenisS Andereya
Nov 28, 2012·Pharmaceutical Research·Claudia Suenderhauf, Neil Parrott
Mar 16, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Adam HatchShashi K Murthy
Jul 20, 2012·Annual Review of Biomedical Engineering·Mónica Beato CoelhoJeffrey M Karp
Jul 26, 2019·Journal of the American Heart Association·Peter V JohnstonGary Gerstenblith
Nov 16, 2017·Current Protocols in Stem Cell Biology·Li Liu, Chien Ho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.