Isolation of plasmids carrying either the uvrC or uvrC uvrA and ssb genes of Escherichia coli K-12

Gene
G H YoakumL Grossman

Abstract

A 3.4 kb PstI fragment containing the uvrC gene of Escherichia coli K-12 has been cloned into pBR322. Plasmids carrying this PstI fragment, in either orientation (pGY3233, pGY4211) relative to the cloning vehicle, complement uvrC mutants. A second plasmid (pGY3243) with a 21 kb HindIII fragment is shown to complement mutations in uvrA and ssb (single-strand binding protein). A composite plasmid (pGY4610) containing pBR322 and PstI fragments derived from pGY3233 (3.4 kb) and pGY3243 (11.05 kb) complements the uvrC, uvrA and ssb mutations.

References

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Citations

Jan 1, 1985·Gene·J W Forster, P Strike
Aug 1, 1982·Biochimie·W D RuppG B Sancar
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S SharmaR E Moses
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A SancarW D Rupp
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G H YoakumL Grossman
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A T YeungL Grossman
Jul 9, 1981·Nature·G H Yoakum, L Grossman
Mar 1, 1988·Molecular & General Genetics : MGG·J W Forster, P Strike
Sep 1, 1988·International Journal of Radiation Biology·J S Rubin
Dec 1, 1990·Microbiological Reviews·R R Meyer, P S Laine

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