Isolation of pMGT1: a gene that is repressed by oestrogen and increased by antioestrogens and antiprogestins

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
D L Manning, R I Nicholson

Abstract

In order to isolate additional markers of oestrogen responsiveness in breast cancer and to study the mechanisms associated with the development of endocrine resistance, we have searched for oestrogen regulated genes. Differential hybridisation analysis of a cDNA library prepared from oestrogen-stimulated T-47D cells has led to the isolation of a sequence (pMGT1) whose expression is repressed (up to 8-fold) by oestrogen (10(-9) mol/l) and represents the first down-regulated gene to be identified by this methodology. Further studies of pMGT1 expression in MCF-7 cells has revealed that the pure antioestrogens, ICI164384 (10(-7) mol/l) and ICI182780 (10(-7) mol/l) and the antiprogestin Ru38486 (10(-7) mol/l), increase pMGT1 mRNA levels by approximately 40-50-fold relative to the value seen in cells exposed to oestrogens. Under the same conditions, pS2(pLIV2), a gene which is positively regulated by oestradiol, was almost undetectable. Significantly, both tamoxifen (10(-7) mol/l), and 4-hydroxytamoxifen (10(-7) mol/l), failed to increase pMGT1 mRNA levels. Since cell culture studies have indicated that ICI164384 and ICI182780 are more effective than tamoxifen and 4-hydroxytamoxifen at inhibiting the growth of MCF-7 cells by mechanis...Continue Reading

References

Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K J WalkerR I Nicholson
Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R I NicholsonR W Blamey
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·A E Wakeling
Oct 1, 1988·Molecular and Cellular Endocrinology·D L ManningC D Green
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Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M C RioP Chambon
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y BerthoisB S Katzenellenbogen

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Citations

Jan 1, 1995·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F E May, B R Westley
Dec 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·A E Wakeling
Aug 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·G VollmerR Knuppen
Apr 30, 1996·Annals of the New York Academy of Sciences·R I NicholsonC K Osborne
Jun 16, 2004·Molecular and Cellular Endocrinology·Sujong KimMi-Ock Lee
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D L ManningR W Blamey
Jun 12, 1995·Annals of the New York Academy of Sciences·R I NicholsonB S Katzenellenbogen
Jan 1, 1995·Acta Oncologica·D L ManningC J Ormandy
Jun 12, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Neill PataniMitch Dowsett
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·D L ManningR I Nicholson
Apr 1, 1997·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·W R Miller, S P Langdon

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European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
D L ManningR I Nicholson
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