Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model.

Science
Thomas F RogersDennis R Burton

Abstract

Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen more than 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. As indicated by maintained weight and low lung viral titers in treated animals, the passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs also define protective epitopes to guide vaccine design.

References

Apr 11, 2001·Advances in Immunology·P W Parren, D R Burton
Aug 17, 2004·Journal of Virology·Tsanan GiroglouDorothee von Laer
Dec 15, 2004·Journal of Virology·Anjeanette RobertsKanta Subbarao
Nov 4, 2008·Nucleic Acids Research·Marie-Paule LefrancPatrice Duroux
Mar 5, 2014·Nature·Nicole A Doria-RoseJohn R Mascola
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Devin SokDennis R Burton
Jun 25, 2015·Microbiology Spectrum·Scott B Halstead
Mar 5, 2016·Nature·Robert N KirchdoerferAndrew B Ward
Aug 26, 2016·PLoS Pathogens·Joseph G JardineWilliam R Schief
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Tushar JainK Dane Wittrup
Jan 31, 2018·Nature Reviews. Immunology·Laura M Walker, Dennis R Burton
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anna Z WecLaura M Walker
Mar 28, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jasper Fuk-Woo ChanKwok-Yung Yuen
May 15, 2020·Cell Host & Microbe·Dennis R Burton, Laura M Walker

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Citations

Jul 22, 2020·The Journal of Experimental Medicine·Fabian SchmidtPaul D Bieniasz
Jul 14, 2020·Nature·Ann M ArvinHerbert W Virgin
Aug 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tyler E MillerJochen K Lennerz
Aug 13, 2020·Nature Reviews. Immunology·Stylianos BournazosJeffrey V Ravetch
Sep 4, 2020·Signal Transduction and Targeted Therapy·Xiangyu ChenLilin Ye
Sep 6, 2020·Disease Models & Mechanisms·Sarah R LeistDavid R Martinez
Jul 30, 2020·The New England Journal of Medicine·Kizzmekia S CorbettBarney S Graham
Jul 15, 2020·Science·Meng YuanIan A Wilson
Sep 6, 2020·Nature Communications·Leo HankeGerald M McInerney
Aug 21, 2020·Mucosal Immunology·M D JohansenP M Hansbro
Aug 28, 2020·Signal Transduction and Targeted Therapy·Francesca FagianiCristina Lanni
Jul 6, 2020·Nature·Gary R Whittaker, Susan Daniel
Oct 6, 2020·Therapeutic Advances in Infectious Disease·Keegan Chua Vi LongYogesh Acharya
Oct 22, 2020·Nature Chemical Biology·Colton J BrackenJames A Wells
Oct 17, 2020·ImmunoTargets and Therapy·Henok AndualemWasihun Hailemichael
Oct 29, 2020·ELife·Yiska WeisblumPaul D Bieniasz
Nov 25, 2020·Current Opinion in HIV and AIDS·Pilar MendozaChristian Gaebler
Oct 13, 2020·Nature·Christopher O BarnesPamela J Bjorkman
Nov 20, 2020·The Journal of Experimental Medicine·Alexandra SchäferTimothy P Sheahan
Dec 3, 2020·Frontiers in Medicine·Maximilian KoblischkeJudith H Aberle
Dec 9, 2020·Science Translational Medicine·Zijun WangMichel C Nussenzweig
Dec 10, 2020·Nature Biomedical Engineering·Shibo JiangLanying Du
Oct 22, 2020·Clinical Chemistry·Raymond T SuhandynataRobert L Fitzgerald
Dec 16, 2020·ELife·Eric Poeschla
Dec 16, 2020·SLAS Discovery·Dawid MaciorowskiAdinarayana Kunamneni
Aug 14, 2020·The Journal of Cell Biology·Fred D MastJohn D Aitchison
Nov 21, 2020·Current Protocols in Immunology·Abigail VanderheidenMehul S Suthar

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Methods Mentioned

BETA
ELISA
PCR
enzyme-linked immunosorbent assay
biolayer interferometry
biosensors
surface plasmon resonance

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