Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients.

Cloning and Stem Cells
Henry KlassenMichael J Young

Abstract

Work in rodents has demonstrated that progenitor transplantation can achieve limited photoreceptor replacement in the mammalian retina; however, replication of these findings on a clinically relevant scale requires a large animal model. To evaluate the ability of porcine retinal progenitor cells to survival as allografts and integrate into the host retinal architecture, we isolated donor cells from fetal green fluorescent protein (GFP)-transgenic pigs. Cultures were propagated from the brain, retina, and corneo-scleral limbus. GFP expression rapidly increased with time in culture, although lower in conjunction with photoreceptor markers and glial fibrillary acid protein (GFAP), thus suggesting downregulation of GFP during differentiation. Following transplantation, GFP expression allowed histological visualization of integrated cells and extension of fine processes to adjacent plexiform layers. GFP expression in subretinal grafts was high in cells expressing vimentin and lower in cells expressing photoreceptor markers, again consistent with possible downregulation during differentiation. Cells survived transplantation to the injured retina of allorecipients at all time points examined (up to 10 weeks) in the absence of exogenou...Continue Reading

References

Jan 1, 1997·Molecular and Cellular Neurosciences·T D PalmerF H Gage
Jul 15, 1999·Biochemical and Biophysical Research Communications·H S LiuN J Ke
Dec 22, 1999·Gene Therapy·F J RubioA Martínez-Serrano
Aug 10, 2000·Nature Medicine·E S Huang, R A Johnson
Aug 13, 2002·Cloning and Stem Cells·D Bart CarterRandall S Prather
Dec 4, 2003·Journal of Neuroscience Research·Philip H SchwartzHenry Klassen
Jan 7, 2004·Biochemical and Biophysical Research Communications·Vikram DevganPolani B Seshagiri
Oct 27, 2004·Investigative Ophthalmology & Visual Science·Henry J KlassenMichael J Young
Aug 16, 2005·Stem Cells·Philip H SchwartzHenry Klassen
Apr 14, 2006·Expert Opinion on Biological Therapy·Henry Klassen
May 9, 2006·Experimental Eye Research·Markus H KuehnYoung H Kwon
Nov 10, 2006·Nature·R E MacLarenR R Ali
Dec 21, 2006·Cell Transplantation·Karin WarfvingeMichael J Young
Jul 7, 2007·Stem Cells·E Scott SwensonDiane S Krause

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Citations

Mar 4, 2011·Reproduction, Fertility, and Development·Jorge A Piedrahita, Natasha Olby
Dec 19, 2012·Reproduction, Fertility, and Development·Monika Nowak-Imialek, Heiner Niemann
Sep 21, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·David D Eveleth
Jul 3, 2009·The Journal of Reproduction and Development·Hitomi Matsunari, Hiroshi Nagashima
Jan 21, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ulrica Englund JohanssonKarin Warfvinge
Jul 10, 2012·Progress in Retinal and Eye Research·Magdalene J Seiler, Robert B Aramant
Jul 4, 2015·Scientific Reports·Elliott H SohnBudd A Tucker
Dec 18, 2008·Molecular Reproduction and Development·Kristin M WhitworthRandall S Prather
Jun 15, 2011·Molecular Reproduction and Development·Jeffrey J Whyte, Randall S Prather
Jun 12, 2013·Veterinary Ophthalmology·Caroline J Zeiss
Apr 16, 2010·The Journal of Comparative Neurology·M Joseph PhillipsDavid M Sherry
May 3, 2012·Stem Cells International·Jens Folke KiilgaardMorten la Cour
May 12, 2012·Stem Cells International·Jing YangHenry Klassen
Apr 11, 2014·BioMed Research International·Girish K SrivastavaJ Carlos Pastor
Nov 26, 2010·Journal of Biomechanics·Niccole M GermscheidKevin A Hildebrand
Oct 21, 2016·The Journal of Reproduction and Development·Masaki NagayaHiroshi Nagashima
Dec 19, 2015·Toxicologic Pathology·Elke-Astrid AtzpodienPeter Maloca
Jun 21, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Monika Nowak-ImialekHeiner Niemann
May 11, 2017·Journal of Translational Medicine·Zhimin TangPing Gu

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Methods Mentioned

BETA
xenografts
transgenic
scraping
dissection

Software Mentioned

Image Pro Plus

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