Isolation of prostacyclin from whole blood

Prostaglandins
V Skrinska, F V Lucas

Abstract

A method was development for the isolation of prostacyclin (PGI2) from whole blood in a fraction suitable for high pressure liquid chromatography (HPLC) separation of PGI2 and 6-keto-prostaglandin F1 alpha (6-K-PGF1 alpha). Prostacyclin was stabilized in whole blood by rapidly raising the pH to 10 with Na2CO3 and cooling the samples to 0 degree C. Under these conditions, 2.9% hydrolysis was observed after 20 min. Reverse phase extraction columns were used to directly extract both PGI2 and 6-K-PGF1 alpha from the alkaline plasma with recoveries of greater than 95% using an acetonitrile/2mM Na2B4O7, pH 10, 40/60 elution solvent mixture. An additional 1.7% hydrolysis was found during the column extraction procedure. Final separation of PGI2 and 6-K-PGF1 alpha was performed with HPLC using an alkaline solvent system. This method is capable of rapidly and efficiently extracting and separating PGI2 and 6-K-PGF1 alpha from whole blood or plasma. It introduces less than 5% hydrolysis of PGI2, thus providing a means of applying highly sensitive 6-K-PGF1 alpha assays to the determination of PGI2 levels in physiological samples.

References

Jun 1, 1978·Prostaglandins·M J Cho, M A Allen
Mar 1, 1977·Prostaglandins·R R GormanO V Miller
Sep 1, 1979·Journal of Chromatography·G T Hill
Sep 1, 1979·Journal of Chromatography·M A WynaldaF A Fitzpatrick
Nov 1, 1980·The Journal of Clinical Investigation·A J MarcusM J Broekman
Feb 1, 1980·Prostaglandins·J O'GradyS Moncada
Nov 1, 1980·Prostaglandins·M A Wynalda, F A Fitzpatrick
Dec 1, 1980·Prostaglandins·R Franco-SaenzS Y Tan
Feb 1, 1981·Prostaglandins·D D PiferC M Chesney

❮ Previous
Next ❯

Citations

Oct 1, 1989·American Journal of Obstetrics and Gynecology·B S StonestreetJ A Widness
Jul 1, 1996·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·T OhtsukaM Arita
May 9, 2012·Drug Design, Development and Therapy·Olivier LambertNagesh Palepu
Oct 11, 2012·Drug Design, Development and Therapy·Olivier Lambert, Dirk Bandilla
May 1, 1983·Journal of Neurosurgery·I AwadR P Lesser
Oct 14, 1983·Journal of Chromatography·V Skrinska, G Thomas
Aug 15, 1986·Thrombosis Research·F V LucasB L Hesse
Jun 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I S McLennan
Mar 1, 1988·JPEN. Journal of Parenteral and Enteral Nutrition·D P KatzJ L Knittle
Mar 1, 1983·Stroke; a Journal of Cerebral Circulation·I AwadR P Lesser
Jul 1, 1991·International Journal of Radiation Biology·V H FingarK W Doak
May 1, 1987·Infection and Immunity·H KawasakiA Tanaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.