Isolation of rainbow trout neutrophils with an anti-granulocyte monoclonal antibody

Veterinary Immunology and Immunopathology
S H HamdaniChristopher J Secombes

Abstract

A magnetic cell sorting system has been optimised for the purification of rainbow trout neutrophils using a monoclonal antibody (E3D9) raised against Atlantic salmon neutrophils. The purified neutrophils have good viability (85%) and purity (approximately 92%), and were functional in respiratory burst and migration assays. The isolated neutrophils responded rapidly to PMA stimulation, producing levels of superoxide anion (4.85 nmols superoxide min-1/10(6) cells) approximately twice as high as macrophages from the same species. In the migration assay, there was a four-fold increase in migrating cells using the purified neutrophils compared with unfractionated blood leucocytes, and a relatively high neutrophil migratory activity was seen in the absence of serum.

References

Jan 1, 1991·Developmental and Comparative Immunology·P R WaterstratG Capley
Sep 1, 1990·Veterinary Immunology and Immunopathology·A J AinsworthT Greenway
Jan 1, 1990·Developmental and Comparative Immunology·J E BlyL W Clem
Jan 1, 1988·Developmental and Comparative Immunology·C J SecombesA H Jeffries
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Jul 1, 1997·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·L A BowdenA F Rowley

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Citations

Jun 9, 2005·Fish & Shellfish Immunology·Uwe FischerTeruyuki Nakanishi
Aug 7, 1999·Veterinary Immunology and Immunopathology·A M O'DowdC J Secombes
Dec 3, 2008·Developmental and Comparative Immunology·Barbara A Katzenback, Miodrag Belosevic
Aug 21, 2007·Veterinary Immunology and Immunopathology·U Fischer, B Koellner
Apr 5, 2002·Fish & Shellfish Immunology·Yurie SasakiNobuaki Okamoto
May 4, 2007·Fish & Shellfish Immunology·Anita RønnesethEirin Fausa Pettersen
Nov 13, 2015·Biology·Jeffrey J Havixbeck, Daniel R Barreda
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hani BoshraJ Oriol Sunyer
Sep 14, 2010·Fish & Shellfish Immunology·Jan H W M RomboutViswanath Kiron

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