PMID: 9185582Jul 1, 1997Paper

Isolation of rare transcripts by representational difference analysis

Nucleic Acids Research
M J O'Neill, A H Sinclair

Abstract

Representational difference analysis (RDA) is a powerful technique for cloning the differences between genomes, and has recently been adapted for cloning differentially expressed genes. RDA, like other PCR-based differential screening methods, is prone to the production of false positives. We have identified a major source of false positives in RDA of cDNA and have introduced improvements which minimise their production. These modifications also significantly increase sensitivity, allowing for the isolation of rare differential transcripts from nanogram amounts of mRNA.

References

Oct 11, 1992·Nucleic Acids Research·J WelshM McClelland
Nov 11, 1995·Nucleic Acids Research·D J BertioliJ F Antoniw
Jun 1, 1995·Trends in Genetics : TIG·M McClellandJ Welsh
Feb 12, 1993·Science·N LisitsynM Wigler

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Citations

Mar 4, 2000·International Journal of Cancer. Journal International Du Cancer·A O GüreY T Chen
Feb 5, 2000·Journal of Neuroscience Research·C ColantuoniJ Pevsner
Jun 23, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dong-Soo ParkJong-Seong Jeon
May 29, 2002·Journal of Microbiological Methods·Andreas Felske
Nov 14, 1997·Current Opinion in Biotechnology·M B Soares
Mar 21, 1998·Current Opinion in Biotechnology·G R Taylor, P Robinson
Mar 10, 2004·Genes, Brain, and Behavior·S KõksE Vasar
Jan 30, 2010·Development·Thiagarajan VenkataramaMary Lou King
Oct 18, 2000·Biochemical and Biophysical Research Communications·S YousefiG Jarai
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S M TannerA de La Chapelle
Sep 3, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Karen E Yates, Julie Glowacki
Aug 9, 2005·Brain Research. Molecular Brain Research·Sueli M Oba-ShinjoMari C Sogayar
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Qi Jun ZhangMartin Bennett
Apr 20, 2000·The Journal of Biological Chemistry·M CervelleraA Sala
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·R Wu, Y Chen
Jan 5, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·D N AryeeH Kovar
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·P CooperG Jarai
Feb 12, 2008·Current Protocols in Molecular Biology·Yuan Chang
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·P CooperG Jarai

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