Isolation of Ready-to-Use Adipose-Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation

Current Protocols in Stem Cell Biology
Edoardo Raposio, Nicolò Bertozzi

Abstract

Current literature does not offer a standardized method to isolate adipose-derived stem cells (ASCs) for clinical applications and hence clinical studies using ASCs often show inconsistent results. Most of these studies borrow laboratory or benchside-derived protocols, which are complex, time consuming, and involve the use of chemical, animal-derived reagents. In this unit we describe a relatively simple and faster isolation protocol that allows collection of a ready-to-use ASC pellet for clinical application. All steps are performed in a closed circuit in order to guarantee maximum process sterility. Once the adipose tissue is harvested by means of a standard liposuction procedure, it undergoes a first centrifugation in order to remove the oil and serous fractions. Then ASCs are released by enzymatic digestion from the surrounding connective tissue scaffold. Finally a double series of washing and centrifugation allows one to obtain the ASC pellet alone. We usually graft this ASC pellet onto the skin edge and to the bottom of chronic skin ulcers as ASCs proved to be effective in promoting wound healing processes. Moreover, an increasing number of clinical studies are currently ongoing to test their potential in every medical fi...Continue Reading

References

Apr 19, 2008·Journal of Cellular and Molecular Medicine·Leonardo A Meza-ZepedaPhilippe Collas
Jun 8, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Feras Al BattahSarah Snykers
Aug 3, 2011·Acta Cirúrgica Brasileira·Leandro FadelCarlos Eduardo Ambrósio
Apr 16, 2013·Plastic and Reconstructive Surgery·Christine FisherJ Peter Rubin
Apr 23, 2013·Food Chemistry·UNKNOWN Food Monitoring Group
Nov 29, 2013·Archives of Plastic Surgery·Ara A SalibianGregory Rd Evans
Dec 24, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Lauren E KokaiJ Peter Rubin
Nov 27, 2014·Journal of Cellular and Molecular Medicine·Derek A BanyardGregory R D Evans
Sep 30, 2015·Annals of Plastic Surgery·Edoardo RaposioMichele P Grieco

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Citations

Oct 17, 2019·Archives of Dermatological Research·Alessia PaganelliCristina Magnoni
May 17, 2018·Stem Cell Research & Therapy·Jens Selch HolmJens Ahm Sorensen
Jul 1, 2018·International Journal of Molecular Sciences·Paola PalumboMaurizio Giuliani
Feb 29, 2020·Regenerative Therapy·Edward H NtegeYusuke Shimizu
Mar 29, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zizhen SiYang Hui
Jul 2, 2019·Cells, Tissues, Organs·Alessia PaganelliCristina Magnoni

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