Isolation of rifampicin resistant Flavobacterium psychrophilum strains and their potential as live attenuated vaccine candidates

Vaccine
B R LaFrentzK D Cain

Abstract

Previous studies have demonstrated that passage of pathogenic bacteria on increasing concentrations of the antibiotic rifampicin leads to the attenuation of virulence and these resistant strains may serve as live attenuated vaccines. Two rifampicin resistant strains of Flavobacterium psychrophilum, 259-93A.16 and 259-93B.17, were generated by passage on TYES plates containing increasing concentrations of rifampicin. Electrophoretic analysis of whole-cell lysates prepared from the parent and resistant strains identified five differentially expressed proteins between the 259-93B.17 strain and parent strain, while there were no differences identified between the 259-93A.16 and parent strain. The LPS banding patterns were identical between all three strains. Bacterial challenges of rainbow trout (Oncorhynchus mykiss Walbaum) with the resistant strains demonstrated that the 259-93B.17 strain was highly attenuated and the 259-93A.16 strain was modestly attenuated at the challenge doses tested. Immunization of rainbow trout with the live attenuated 259-93B.17 strain by intraperitoneal injection resulted in significant protection against challenge with the virulent parent F. psychrophilum strain at 8 and 15 weeks post-immunization and ...Continue Reading

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Citations

Jul 25, 2014·Microbial Biotechnology·Esther GómezJosé A Guijarro
Jul 13, 2016·Fish & Shellfish Immunology·Ponnerassery S Sudheesh, Kenneth D Cain
Nov 10, 2010·Fish & Shellfish Immunology·Craig A ShoemakerJoyce J Evans
Oct 12, 2013·Diseases of Aquatic Organisms·Lingyu GuanRoy Curtiss
Jun 14, 2020·Applied and Environmental Microbiology·Paul BarbierMark J McBride

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