PMID: 8589412Jul 1, 1995Paper

Isolation of ropB, a gene encoding a 22-kDa Rhizobium leguminosarum outer membrane protein

Molecular Plant-microbe Interactions : MPMI
H P RoestB J Lugtenberg

Abstract

As judged from immunochemical detection, the levels of outer membrane antigen groups II and III of Rhizobium leguminosarum bv. viciae strain 248 decrease during bacteroid differentiation (R. A. de Maagd, R. de Rijk, I. H. M. Mulders, and B. J. J. Lugtenberg, J. Bacteriol. 171:1136-1142, 1989). Using a newly developed colony blot assay, a cosmid clone expressing the Mab8 epitope of the outer membrane antigen group II of R. l. bv. viciae strain 248 was selected in Rhizobium meliloti LPR2120. From this cosmid the gene encoding this epitope was cloned and characterized. An open reading frame of 636 nucleotides was found and predicted to encode a protein with a calculated molecular mass of 22.5 kDa. After subtraction of the predicted 23 amino acid signal peptide, a M(r) of 20.3 kDa was calculated for the mature protein. This gene, designated ropB, was not active in Escherichia coli under the control of its own promoter. The C-terminal amino acid of the protein is a phenylalanine residue which is required for efficient translocation of outer membrane proteins. Membrane spanning amphipathic beta-sheets are predicted to represent a major part of the secondary structure of the protein. A model of the topology of the protein is presented...Continue Reading

Citations

Oct 27, 2004·Archives of Microbiology·Christopher K YostMichael F Hynes
Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer
Nov 5, 2002·Veterinary Microbiology·I López-GoñiE Moreno
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·C Guzman-VerriI Lopez-Goni
Jun 19, 2003·Journal of Bacteriology·Gordon R O CampbellGraham C Walker
Nov 7, 2019·Biomolecules·Anastasiia O KosolapovaAnton A Nizhnikov

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