PMID: 9535160Apr 16, 1998Paper

Isolation of seven unique biogenic amine receptor clones from the honey bee by library scanning

Insect Molecular Biology
Paul R EbertD P Toma

Abstract

The biogenic amine receptor genes constitute an ancient and highly divergent family within the larger superfamily of G-protein-coupled receptors. These receptors play a central role in modulating nerve cell activity and thus behaviour. Because the honey bee offers numerous advantages for behavioural studies we endeavoured to isolate as many members of this gene family as possible from the bee. We compared numerous approaches to gene isolation and found that PCR amplification from small subfractions of cDNA or genomic DNA libraries enabled us to isolate clones that are otherwise undetectable. In total we isolated seven biogenic amine receptor clones and identified five additional related sequences by low-stringency Southern hybridization. Two clones, AmBAR4 and AmBAR6, are 84% and 72% identical to the Drosophila 5-HT2 and D1b receptors, respectively, and probably represent orthologous genes. Phylogenetic analysis indicates that AmBAR5 clusters loosely with a variety of tyramine and octopamine receptors with which it shares <66% identity. The other four clones, AmBAR1, AmBAR2, AmBAR3 and AmBAR7, are weakly to moderately related (28-45% identical) to Drosophila dopaminergic or mammalian adrenergic receptors and probably represent ...Continue Reading

Citations

May 17, 2003·Brain Research. Molecular Brain Research·Julie A MustardAlison R Mercer
Jun 30, 2005·Archives of Insect Biochemistry and Physiology·Julie A MustardAlison R Mercer
Feb 24, 2004·Journal of Neurobiology·Sudipta SaraswatiChun-Fang Wu
Jun 10, 2005·Insect Biochemistry and Molecular Biology·Kyle T BeggsAlison R Mercer
May 8, 2008·Journal of Economic Entomology·Vincent Le RouxPhilippe Giordanengo
Jun 27, 2003·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·M A HumphriesR E Page

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