Isolation of skin dendritic cells from mouse and man

Methods in Molecular Biology
Patrizia StoitznerSusanne Ebner

Abstract

Dendritic cells (DC) are crucial for the induction of immune responses and populate various tissues to fulfil their special role. The skin harbours different DC subsets, the Langerhans cells (LC) in the epidermis and the dermal DC in the dermis. The investigation of skin DC is cumbersome since these cells are rare in the skin. As a consequence, it is laborious to receive enough cells from the tissue for experiments. Several approaches have been developed to isolate skin DC based on either enzymatic digestion of the tissue or skin explant culture. Immature LC can be obtained by trypsinization of epidermis, cultured in vitro and be highly enriched with gradient centrifugation and magnetic bead sorting. Mature skin DC can be easily received from skin explant culture. For this purpose skin pieces are cultured for several days and migratory DC emigrate from epidermis and dermis. Both techniques are described for human and mouse skin in the following chapter of the book.

Citations

Aug 23, 2016·Nature Immunology·Ji Hyung KimFlorian Winau
May 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucille AdamFrédéric Martinon
Dec 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nancy LuckashenakThomas Brocker
Jul 25, 2018·Current Protocols in Immunology·Sakeen W Kashem, Daniel H Kaplan
Jul 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Olivier EpaulardFrédéric Martinon
May 20, 2014·European Journal of Immunology·Katharina LahlEugene Butcher
Jun 14, 2018·Cancer Immunology Research·Christiane QuerfeldJames W Young
Apr 3, 2021·Experimental Dermatology·Christoph H TrippPatrizia Stoitzner
May 30, 2019·ACS Central Science·Eike-Christian WamhoffChristoph Rademacher
Sep 5, 2021·Nature Protocols·Marcela Alcántara-Hernández, Juliana Idoyaga
Dec 22, 2021·European Journal of Immunology·Lydia BellmannPatrizia Stoitzner

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