PMID: 7541542Jul 3, 1995Paper

Isolation of small polarized bile duct units

Proceedings of the National Academy of Sciences of the United States of America
A MennoneJ L Boyer

Abstract

Fragments of small interlobular bile ducts averaging 20 microns in diameter can be isolated from rat liver. These isolated bile duct units form luminal spaces that are impermeant to dextran-40 and expand in size when cultured in 10 microM forskolin for 24-48 hr. Secretion is Cl- and HCO3- dependent and is stimulated by forskolin > dibutyryl cAMP > secretion but not by dideoxyforskolin, as assessed by video imaging techniques. Secretin stimulates Cl-/HCO3- exchange activity, and intraluminal pH increases after forskolin administration. These studies establish that small polarized physiologically intact interlobular bile ducts can be isolated from rat liver. These isolated bile duct units should be useful preparations for assessing the transport properties of small bile duct segments, which are the primary site of injury in cholestatic liver disorders, known as "vanishing bile duct syndromes."

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Citations

Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TraunerJ L Boyer
Aug 21, 2003·Journal of Hepatology·Petra HolmströmGösta Eggertsen
Feb 12, 1998·Journal of Hepatology·M Strazzabosco
Jul 29, 1999·Journal of Hepatology·L BaiocchiG Alpini
Apr 25, 2001·Liver·G LeSageG Alpini
Aug 4, 2001·The Biochemical Journal·M H NathansonN F Larusso
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R B DoctorJ G Fitz
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D AlvaroG Alpini
May 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Noriatsu KannoGianfranco Alpini
Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Gene D LesageGianfranco Alpini
Sep 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hélène Strick-Marchand, Mary C Weiss
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S F SchlosserM H Nathanson
Feb 24, 2006·Biochemistry. Biokhimii︠a︡·R L BogoradO V Smirnova
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin YuJonathan A Dranoff
Jul 20, 2012·American Journal of Physiology. Renal Physiology·Xiaofang WangVicente E Torres
Sep 18, 1997·The Journal of Clinical Investigation·D AlvaroA Benedetti
Jun 24, 1998·The Journal of Clinical Investigation·M H NathansonL Rios-Velez
May 29, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Emina HalilbasicMichael Trauner
May 16, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mario StrazzaboscoGianfranco Alpini
Feb 27, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·James L Boyer
Dec 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Iker UriarteJuan F Medina
Jan 25, 2005·Journal of Hepatology·Marc BeaussierChantal Housset
Jul 1, 1997·The Journal of Laboratory and Clinical Medicine·A P Kwiatkowski, J M McGill
Dec 20, 2002·Mechanisms of Development·Hélène Strick-Marchand, Mary C Weiss
Jan 1, 1996·Toxicologic Pathology·N F LaRusso
Apr 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R B DoctorJ G Fitz
Dec 19, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Carol J SorokaJames L Boyer
Jan 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jesús M BanalesJuan F Medina
Nov 10, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Biyi ChenWon Kyoo Cho
May 30, 2002·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Emma A KruglovJonathan A Dranoff
Dec 31, 1997·The American Journal of Physiology·T SchlenkerJ G Fitz
Mar 11, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·K D SalterR M Roman
Jan 24, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gianfranco AlpiniGene LeSage
Nov 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Won Kyoo Cho
Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·G LesageG Alpini

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