Isolation of special antibodies which react only with homologous enterotoxins from Vibrio cholerae and Enterotoxigenic Escherichia coli.

Infection and Immunity
T HondaT Miwatani

Abstract

Cholera enterotoxin (CT) and heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli share a common antigenic determinant. In addition, CT and LT each have a unique antigenic determinant. Antisera were prepared by immunoaffinity chromatography against these unique antigenic determinants, that is, antiserum that reacted with CT but not with LT and antiserum that reacted with LT but not with CT. Antiserum against the common antigenic determinant to CT and LT was also prepared by immunoaffinity chromatography. The specificities of these antisera were demonstrated by Ouchterlony double-gel diffusion tests and by neutralization of the activities of CT and LT to cause morphological changes in Chinese hamster ovary cells.

References

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Citations

Jan 1, 1988·Microbiology and Immunology·Y OkuY Takeda
Jan 15, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yoshifumi Takeda
Dec 1, 1983·Microbiological Reviews·L EidelsD A Hart
Jan 1, 1985·Pharmacology & Therapeutics·D C RobertsonF Dorner
Nov 1, 1987·Annales De L'Institut Pasteur. Microbiology·A C SaidA F Pestana de Castro
Nov 1, 1987·Journal of Bacteriology·A K ChopraJ J Mekalanos

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