Isolation of Squarebop I bacteriorhodopsin from biomass of coastal salterns

Protein Expression and Purification
Simona LobassoAngela Corcelli

Abstract

Squarebop I bacteriorhodopsin is a light-activated proton pump present in the membranes of the archeon Haloquadratum walsbyi, a square-shaped organism representing 50-60% of microbial population in the crystallizer ponds of the coastal salterns. Here we describe: (1) the operating mode of a bioreactor designed to concentrate the saltern biomass through a microfiltration process based on polyethersulfone hollow fibers; (2) the isolation of Squarebop I bacteriorhodopsin from solubilized biomass by means of a single chromatographic step; (3) tightly bound lipids to the isolated and purified protein as revealed by MALDI-TOF/MS analysis; (4) the photoactivity of Squarebop I bacteriorhodopsin isolated from environmental samples by flash spectroscopy. Yield of the isolation process is 150 μg of Squarebop I bacteriorhodopsin from 1l of 25-fold concentrated biomass. The possibility of using the concentrated biomass of salterns, as renewable resource for the isolation of functional bacteriorhodopsin and possibly other valuable bioproducts, is briefly discussed.

References

Dec 1, 1991·Photochemistry and Photobiology·Y MukohataY Sugiyama
Mar 30, 1988·Biochemical and Biophysical Research Communications·Y MukohataM Yoshida
Sep 29, 1971·Nature: New Biology·D Oesterhelt, W Stoeckenius
Dec 4, 2003·Environmental Microbiology·R Thane PapkeFrancísco Rodríguez-Valera
Sep 11, 2004·FEMS Microbiology Letters·David G BurnsMike L Dyall-Smith
Nov 25, 2004·Environmental Microbiology·Henk BolhuisFrancisco Rodriguez-Valera
Apr 5, 2005·Archaea : an International Microbiological Journal·Chiara SchiraldiMario De Rosa
Dec 5, 2008·Archaea : an International Microbiological Journal·Simona LobassoAngela Corcelli
Mar 10, 2010·Journal of Lipid Research·Maristella BaronioAngela Corcelli
Jun 12, 2010·Journal of Lipid Research·Roberto AngeliniAngela Corcelli
Jul 29, 2010·Environmental Technology·Aharon Oren
Jun 28, 2011·PloS One·Mike L Dyall-SmithDieter Oesterhelt
Jan 18, 2012·Photochemistry and Photobiology·Simona LobassoAngela Corcelli
Dec 5, 2012·Archaea : an International Microbiological Journal·Kimberly M Webb, Jocelyne DiRuggiero
Jan 1, 1995·World Journal of Microbiology & Biotechnology·A Ventosa, J J Nieto

❮ Previous
Next ❯

Citations

Oct 29, 2015·Extremophiles : Life Under Extreme Conditions·Aharon OrenRael Horwitz
Jun 14, 2014·Frontiers in Microbiology·Bonnie K BaxterAharon Oren
Jul 5, 2017·BMC Genomics·Henk BolhuisFrancisco Rodriguez-Valera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved