Isolation of Stem Cells and Progenitors from Mouse Epidermis

Current Protocols in Stem Cell Biology
Lana KosticYaron Fuchs

Abstract

The epidermis consists of several distinct compartments including the interfollicular epidermis (IFE), sweat glands, sebaceous glands (SGs), and the hair follicle (HF). While the IFE and SGs are in a constant state of self-renewal, the HF cycles between phases of growth, destruction, and rest. The hair follicle stem cells (HFSCs) that fuel this perpetual cycle have been well described and are located in a niche termed the bulge. These bulge SCs express markers such as CD34 and Keratin 15 (K15), enabling the isolation of these cells. Here, we describe a powerful method for isolating HFSCs and epidermal progenitors from mouse skin utilizing fluorescence activated cell-sorting (FACS). Upon isolation, cells can be expanded and utilized in various in vivo and in vitro models aimed at studying the function of these unique cells. © 2017 by John Wiley & Sons, Inc.

References

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May 21, 2015·Nature Reviews. Molecular Cell Biology·Yaron Fuchs, Hermann Steller
May 12, 2016·Journal of Visualized Experiments : JoVE·Despina SoteriouYaron Fuchs

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Citations

Nov 13, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Yaqin ZhangFuqiu Li
May 30, 2019·Nature Communications·Alona FeldmanYaron Fuchs

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