Isolation of subtelomeric sequences of porcine chromosomes for translocation screening reveals errors in the pig genome assembly

Animal Genetics
R E O'ConnorD K Griffin

Abstract

Balanced chromosomal aberrations have been shown to affect fertility in most species studied, often leading to hypoprolificacy (reduced litter size) in domestic animals such as pigs. With an increasing emphasis in modern food production on the use of a small population of high quality males for artificial insemination, the potential economic and environmental costs of hypoprolific boars, bulls, rams etc. are considerable. There is therefore a need for novel tools to facilitate rapid, cost-effective chromosome translocation screening. This has previously been achieved by standard karyotype analysis; however, this approach relies on a significant level of expertise and is limited in its ability to identify subtle, cryptic translocations. To address this problem, we developed a novel device and protocol for translocation screening using subtelomeric probes and fluorescence in situ hybridisation. Probes were designed using BACs (bacterial artificial chromosomes) from the subtelomeric region of the short (p-arm) and long (q-arm) of each porcine chromosome. They were directly labelled with FITC or Texas Red (p-arm and q-arm respectively) prior to application of a 'Multiprobe' device, thereby enabling simultaneous detection of each in...Continue Reading

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Citations

Jan 16, 2020·Animals : an Open Access Journal From MDPI·Rebecca L JenningsRebecca E O'Connor
Jul 19, 2020·Animals : an Open Access Journal From MDPI·Barbara Danielak-CzechMarek Babicz
Feb 9, 2019·Biochemical Society Transactions·Katie E FowlerDarren K Griffin
Sep 15, 2017·Animal Genetics
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Izabela Szczerbal, Marek Switonski
May 1, 2021·Animals : an Open Access Journal From MDPI·Brendan DonaldsonW Allan King
Jul 13, 2021·Journal of Veterinary Research·Dorota Banaszewska, Katarzyna Andraszek
Sep 4, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Krzysztof GórskiLeyland Fraser

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Methods Mentioned

BETA
Genome Sequencing

Software Mentioned

smarttype
Sscrofa
karyolab porc
Cytocell

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