PMID: 8441663Feb 11, 1993Paper

Isolation of telomeric DNA from the filamentous fungus Podospora anserina and construction of a self-replicating linear plasmid showing high transformation frequency

Nucleic Acids Research
J P JaverzatC Barreau

Abstract

It has been previously shown that linear plasmids bearing Tetrahymena telomeric sequences are able to replicate autonomously in the filamentous fungus Podospora anserina (1). However, autonomous replication occurs in only 50-70% of the transformants, suggesting a defect in the recognition of the Tetrahymena telomeric template by the putative P. anserina telomerase so that only a fraction of entering DNA is stabilized into linear extrachromosomal molecules. We have cloned DNA sequences added to the Tetrahymena (T2G4)n ends of the linear plasmid. Nucleotide sequencing showed that these sequences are exclusively composed of T2AG3 repeat units. Hybridization experiments of Bal31 treated DNA showed that T2AG3 repeats are confined within 200 bp in chromosomal P. anserina telomeres. A new plasmid has been constructed so that after linearization, the terminal sequences contain T2AG3 repeats. This linear molecule transforms P. anserina with a high frequency (up to 1.75 x 10(4) transformants/micrograms), autonomous replication occurs in 100% of the transformants and the plasmid copy number is about 2-3 per nucleus. These results underscore the importance of the telomeric repeat nucleotide sequence for efficient recognition as functional ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 2, 1990·Science·D Shippen-Lentz, E H Blackburn
Apr 18, 1991·Nature·E H Blackburn
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C FarrH Cooke
Apr 27, 1989·Nature·S H CrossH J Cooke
Apr 27, 1989·Nature·W R Brown
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K M HahnenbergerL Clarke
Jan 24, 1989·Biochemistry·C M Price, T R Cech
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L A KlobutcherD M Prescott
Apr 5, 1981·Journal of Molecular Biology·A OkaM Takanami
May 1, 1982·Cell·J W Szostak, E H Blackburn
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A F PlutaV A Zakian
Jul 12, 1984·Nature·J ShampayE H Blackburn
Sep 15, 1983·Nature·A W Murray, J W Szostak
Jan 1, 1984·Annual Review of Biochemistry·E H Blackburn

❮ Previous
Next ❯

Citations

May 1, 1995·Journal of Industrial Microbiology·P A Lemke
Jan 1, 1996·Current Genetics·M D Garcia-Pedrajas, M I Roncero
Sep 26, 1996·Gene·J AvalosS Brenner
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R J Wellinger, D Sen
Sep 10, 2008·Antimicrobial Agents and Chemotherapy·Sabine FillingerDanièle Debieu
May 8, 2008·Genome Biology·Eric EspagnePhilippe Silar
Apr 17, 2003·Applied Microbiology and Biotechnology·K-I KusumotoY Kashiwagi
Nov 28, 2000·Annual Review of Genetics·M J McEachernE H Blackburn
Dec 4, 2010·Fungal Genetics and Biology : FG & B·Jawad MerhejChristian Barreau
Aug 1, 1997·Fungal Genetics and Biology : FG & B·H C Kistler
Jan 22, 1998·FEMS Microbiology Letters·C LevisY Brygoo
Dec 8, 1995·Science·V A Zakian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.