PMID: 6990421Apr 1, 1980Paper

Isolation of the centromere-linked CDC10 gene by complementation in yeast

Proceedings of the National Academy of Sciences of the United States of America
L Clarke, J Carbon

Abstract

A hybrid plasmid colony bank was constructed in Escherichia coli using the E. coli-Saccharomyces cerevisiae shuttle vector pLC544 and randomly sheared segments of yeast DNA. By transformation with a hybrid plasmid DNA pool from this collection and complementation of a temperature-sensitive cdc10 mutation in yeast, a plasmid was isolated that carries 8 kilobase pairs of DNA around the chromosome III centromere-linked CDC10 locus. This DNA segment overlaps a larger region of DNA (40 kilobase pairs) previously identified to be around the LEU2 locus on chromosome III [Chinault, A.C. & Carbon, J. (1979) Gene 5, 111-126] and physically establishes the directionality of the cloned DNA sequences with respect to the genetic map and the centromere. In the leu2-cdc10 interval, the relationship between physical distance on the DNA and genetic distance as measured by recombinational frequencies is about 3 kilobase pairs per centimorgan.

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Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
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Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L Clarke, J Carbon
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K A Nasmyth, S I Reed

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Citations

Jan 1, 1985·Molecular & General Genetics : MGG·C I White, S G Sedgwick
May 1, 1995·Journal of Industrial Microbiology·P A Lemke
Sep 10, 2010·Journal of Biosciences·Amit Bardhan
Aug 1, 1983·Gene·G Tschumper, J Carbon
Aug 24, 2010·Nature Cell Biology·Xue Han, Martin Srayko
Jan 2, 2007·Nature Structural & Molecular Biology·Ronnie R WeiStephen C Harrison
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M RoseD Botstein
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C L Hsiao, J Carbon
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Laughon, R F Gesteland
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G Tschumper, J Carbon
Jan 25, 1983·Nucleic Acids Research·J C Larkin, J L Woolford
Nov 8, 2008·Science·David AltshulerEric S Lander
Sep 13, 2011·Eukaryotic Cell·Babhrubahan Roy, Kaustuv Sanyal
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ronnie R WeiStephen C Harrison
Jun 25, 1982·Journal of Molecular Biology·D T StinchcombR W Davis
Feb 24, 2009·BMC Evolutionary Biology·Elissavet NikolaouAlistair J P Brown
Feb 5, 1981·Journal of Molecular Biology·A J KingsmanJ Carbon
Sep 5, 1983·Nature·K Struhl
Sep 30, 1995·Yeast·M D Kaytor, D M Livingston
Sep 1, 1987·Yeast·M Fitzgerald-Hayes
Feb 12, 2020·Experimental Cell Research·Jun-Ichirou OhzekiHiroshi Masumoto
Feb 1, 1988·Molecular and Cellular Biology·K M Felsenstein, S W Emmons
Dec 1, 1996·Molecular and Cellular Biology·D J MaurerD M Livingston

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