PMID: 2106668Feb 11, 1990Paper

Isolation of the chicken middle-molecular weight neurofilament (NF-M) gene and characterization of its promoter

Nucleic Acids Research
D ZopfE D Gundelfinger

Abstract

We have isolated and sequenced genomic DNA clones covering the coding region of the chicken mid-size neurofilament (NF-M) gene and greater than 1 kb of its 5' upstream region. The NF-M gene contains two introns which both are located within the highly conserved C-terminal region of the rod domain. The 5' end of the corresponding mRNA was assigned to a G residue 40 nucleotides upstream of the translation start site and in appropriate distance from a potential TATA box. To functionally analyze the NF-M promoter, constructs carrying 112, 222, and 1026 nucleotides of the 5' upstream region in front of a luciferase reporter gene were tested for their capability to direct luciferase expression after transient transfection into various cell lines. Significant luciferase activity was recorded both in rat phaeochromocytoma (PC12) cells and murine fibroblasts. In PC12 cells, in which neurite outgrowth is induced by nerve growth factor (NGF), expression was stimulated up to 13-fold within 3 days of NGF treatment. This closely resembles expression of the endogenous NF-M gene in response to this hormone.

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Citations

Oct 11, 1991·Brain Research. Molecular Brain Research·D S HowlandL J DeGennaro
Mar 1, 1992·Brain Research. Molecular Brain Research·P S ShneidmanW W Schlaepfer
Sep 11, 1992·Brain Research. Molecular Brain Research·G A ElderR A Lazzarini
Sep 1, 1992·Brain Research. Molecular Brain Research·G A ElderR A Lazzarini
Apr 1, 1993·Brain Research. Molecular Brain Research·L BeaudetJ P Julien
Oct 23, 1997·Brain Research. Molecular Brain Research·M L SchwartzW W Schlaepfer
Sep 19, 2009·Journal of Biochemistry·Kunio KosakaTakuji Shirasawa
Feb 11, 1993·Nucleic Acids Research·K YazdanbakhshM Lindenbaum
Nov 1, 1995·Journal of Neurogenetics·R M Twyman, E A Jones
Jul 2, 2003·Journal of Neuroscience Research·Ada Maria TataGabriella Augusti-Tocco
Mar 1, 2009·Integrative Zoology·Allen G Strickler, William R Jeffery
Sep 21, 2005·Journal of Cellular Biochemistry·Pei WangChung-Liang Chien
Apr 6, 2001·Journal of Neurobiology·K KosJ N Coulombe
Sep 28, 2017·Chromosoma·Aleksey S KomissarovAlsu F Saifitdinova
Dec 22, 1995·The Journal of Biological Chemistry·G CharronJ P Julien
Aug 9, 1996·Science·S TeradaN Hirokawa

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