Isolation of the genome sequence strain Mycobacterium avium 104 from multiple patients over a 17-year period

Journal of Clinical Microbiology
Kathleen L HoranGerard A Cangelosi

Abstract

The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, ...Continue Reading

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Citations

Aug 9, 2012·The Journal of Antimicrobial Chemotherapy·Rok Kopinc, Ales Lapanje
Jul 16, 2013·Applied and Environmental Microbiology·M Ashworth DiracGerard A Cangelosi
Dec 2, 2009·Applied and Environmental Microbiology·Gerard A CangelosiJohn S Meschke
Jan 5, 2008·Journal of Clinical Microbiology·Maegan AshworthGerard A Cangelosi
Dec 26, 2015·BMC Genomics·Benjamin J GarciaMichael Strong
Oct 9, 2012·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Muhammad Salman AshrafKeith M Ramsey
Feb 25, 2015·BMC Evolutionary Biology·Oleg RevaAleksandr Ilin
Jul 17, 2015·BioMed Research International·Milena Maya-HoyosCarlos Y Soto
Feb 19, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laura RindiCarlo Garzelli
Jun 15, 2007·FEMS Microbiology Letters·Martha I MurciaM Carmen Menéndez
May 31, 2017·Future Microbiology·Rohini MattooGyanu Lamichhane
Oct 27, 2020·Frontiers in Microbiology·Harley ParkerPetros C Karakousis
Jan 14, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shymaa EnanySohkichi Matsumoto

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