Isolation of the interacting molecules with GEX-3 by a novel functional screening

Biochemical and Biophysical Research Communications
Daisuke TsuboiKozo Kaibuchi

Abstract

To screen for important molecules that interact with a gene of interest in Caenorhabditis elegans (C. elegans), we established a novel functional screening system using the yeast two-hybrid system with the RNA interference technique. Our screening system makes it possible to identify the molecular machinery involved in the function of a gene of interest starting with the cDNA of this gene. As a model case, we examined the molecular machinery involved in the function of GEX-3, an essential factor of tissue morphogenesis. We identified many interacting molecules by yeast two-hybrid screening and could detect some functional interactions using this novel functional screening system.

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Nov 17, 2004·Genes & Development·Peter W LewisMichael R Botchan
Oct 31, 2008·BMC Bioinformatics·Matthew T WeirauchJoshua M Stuart
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