Isolation of the outer membrane of Branhamella catarrhalis

Microbial Pathogenesis
T F Murphy, M R Loeb

Abstract

The emergence of Branhamella catarrhalis as an important human pathogen has stimulated interest in investigations of the outer membrane (OM) of the bacterium. In this study, the OM of B. catarrhalis was isolated and partially characterized. Radiolabelled cells were lysed and fractionated by isopycnic centrifugation in a continuous sucrose gradient. Five fractions were identified. Fraction A consisted of OM fragments of varying density. Fractions B and C were OM of a discrete density containing some cytoplasmic membrane. Fraction D was cytoplasmic membrane and Fraction E contained smaller less dense fragments of cytoplasmic membrane. The protein composition of the Branhamella OM is typical for that of Gram-negative bacteria in that approximately 10 to 20 proteins were present with six to eight of these proteins predominating. Having isolated and partially characterized the OM by sucrose density centrifugation, five simpler techniques for isolating OM were employed and the preparations compared to OM isolated on the gradient. Techniques that are based on differential detergent solubility of OM and cytoplasmic membrane were ineffective in isolating OM of B. catarrhalis. By contrast, techniques that involved collection of OM vesicl...Continue Reading

References

Jun 1, 1978·The Journal of Pediatrics·P A ShurinJ O Klein
Jan 1, 1976·Scandinavian Journal of Infectious Diseases·J E BrorsonS E Holm
Jan 1, 1977·Annals of Internal Medicine·D DouerJ Pinkhas
Aug 1, 1976·Annals of Internal Medicine·A A Pollock, R S Holzman
Nov 1, 1987·Reviews of Infectious Diseases·H HagerS L Berk
Oct 1, 1988·The Journal of Infectious Diseases·L C Bartos, T F Murphy
Apr 1, 1987·Australian and New Zealand Journal of Medicine·J H O'Neill, P W Mathieson
Jan 1, 1987·Reviews of Infectious Diseases·G F Van HareC H Kim
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·G V Doern
Jan 1, 1987·The Pediatric Infectious Disease Journal·G F Van Hare, P A Shurin
Mar 1, 1985·Acta Oto-laryngologica·P KarmaP Grönroos
May 1, 1985·The Journal of Infectious Diseases·A J ChapmanR J Wallace
Jul 1, 1974·Journal of Bacteriology·K H Johnston, E C Gotschlich
Oct 1, 1967·Archives of Otolaryngology·J D CoffeyH N Booth
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
May 1, 1983·The Journal of Infectious Diseases·T F MurphyM A Apicella
May 1, 1981·The Journal of Infectious Diseases·S J BarenkampD M Granoff
Sep 1, 1982·Journal of Bacteriology·N KatsuiI Shibasaki

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Citations

Aug 26, 1998·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K McGregorT V Riley
Oct 14, 1998·FEMS Microbiology Letters·G M MaluszynskaT Sundqvist
Nov 26, 1999·The Pediatric Infectious Disease Journal·K MathersD Goldblatt
Mar 14, 2003·The Pediatric Infectious Disease Journal·Patricia Stutzmann MeierChristoph Aebi
Jan 1, 1990·Microbiology and Immunology·K AhmedK Matsumoto
Mar 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M RahmanA Krook
Jan 10, 2002·Clinical Microbiology Reviews·Cees M VerduinAlex van Belkum
Jun 15, 2007·Infection and Immunity·Melanie M Pearson, Eric J Hansen
Mar 26, 2008·Infection and Immunity·Ahmed S AttiaEric J Hansen
Nov 26, 2003·Infection and Immunity·Patricia Stutzmann MeierChristoph Aebi
Mar 25, 2004·Infection and Immunity·Melissa M HolmEric R Lafontaine
Nov 22, 2005·Infection and Immunity·Nadja HeinigerChristoph Aebi
Dec 11, 2007·Journal of Bacteriology·Todd C HoopmanEric J Hansen
Oct 22, 2013·Journal of Bacteriology·Sean W Buskirk, Eric R Lafontaine
May 14, 1990·The American Journal of Medicine·T F Murphy
Nov 30, 2005·Archives of Medical Research·Shankar Esaki MuthuUsha Anand Rao
Nov 20, 2008·Current Protocols in Microbiology·Rachel Balder, Eric R Lafontaine
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Therése NordströmKristian Riesbeck
Jul 1, 1997·FEMS Immunology and Medical Microbiology·M FitzgeraldT Scott

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