Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography

Preparative Biochemistry
T I Mercado, K Katusha

Abstract

Trypomastogotes of three strains of Trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of DEAE cellulose for removal of white cells and platelets. An average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. Protein and carbohydrate assays of the separated organisms revealed significant differences between the Tulahuen, a reticulotropic strain, and the House 510 and House 11, two myotropic strains of this parasitic species. The present procedure should provide sufficient parasites for physiological and biochemical studies; it has also served to indicate particular strain characteristics which may aid in a taxonomic classification of these organisms.

References

May 1, 1975·The Journal of protozoology·J KloetzelV L Giovannini
Dec 1, 1976·Experimental Parasitology·T I Mercado
Jan 1, 1973·Annual Review of Microbiology·Z Brener
Dec 1, 1970·Experimental Parasitology·S M Lanham, D G Godfrey

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Citations

Jan 1, 1990·Parasitology Research·M Q XieL R McDougald
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·W E Gutteridge
Nov 1, 1985·Molecular and Biochemical Parasitology·M C Connelly, F Kierszenbaum
Apr 1, 1985·Molecular and Biochemical Parasitology·J J Wirth, F Kierszenbaum
Feb 1, 1987·Molecular and Biochemical Parasitology·A Ayala, F Kierszenbaum
Jul 1, 1994·Molecular and Biochemical Parasitology·M A YakubuF Kierszenbaum
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F KierszenbaumA Sjoerdsma
May 1, 1993·Parasite Immunology·H M LopezM B Sztein
Dec 1, 1982·Antimicrobial Agents and Chemotherapy·T I Mercado, A Colón-Whitt
Feb 1, 1984·The Journal of protozoology·D M SchmatzP K Murray
Nov 1, 1985·Revista Do Instituto De Medicina Tropical De São Paulo·T Langenbach
Feb 4, 2003·Anais Da Academia Brasileira De Ciências·Thaïs Souto-Padrón

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