Isolation of tube precipitin antibody-reactive fractions of Coccidioides immitis.

Infection and Immunity
D Kruse, G T Cole

Abstract

Patients presenting with primary coccidioidal infection have been shown by earlier investigators to produce immunoglobulin M (IgM) precipitin antibodies to lysates of mycelial and spherule phases of Coccidioides immitis. This humoral response has been detected by tube precipitin (TP) and immunodiffusion (ID)-TP assays of patient sera, which are valuable aids in early diagnosis of coccidioidomycosis. Several reports of antigenic fractions which show reactivity with patient TP antibody have been published. However, confusion persists with respect to the nature of the specific serologically reactive macromolecule(s). In this study we isolated two TP antibody-reactive antigens (TP-Ags) from an alkali-soluble, water-soluble fraction of the inner conidial wall and a culture filtrate plus toluene lysate of the mycelial phase of C. immitis. The crude antigens were first separated by concanavalin A (ConA) chromatography. The TP-Ags were identified in ID-TP assays as 120- and 110-kilodalton (kDa) fractions which were electroeluted from reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis separations of the ConA-bound conidial wall extract and ConA-bound culture filtrate plus lysate preparation, respectively. Following elect...Continue Reading

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Citations

Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·L De RepentignyR C Matthews
Jul 1, 1991·Infection and Immunity·K O DuggerJ H Law
Nov 1, 1991·Infection and Immunity·T N KirklandG T Cole
Jul 1, 1990·Clinical Microbiology Reviews·D Pappagianis, B L Zimmer
Aug 24, 2010·Autoimmunity Reviews·Andrea T Borchers, M Eric Gershwin

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