PMID: 8580966Dec 1, 1995Paper

Isolation of two novel myb-like genes from Arabidopsis and studies on the DNA-binding properties of their products

The Plant Journal : for Cell and Molecular Biology
S F Li, R W Parish

Abstract

Two novel myb-like genes (atmyb6 and atmyb7) were isolated from an Arabidopsis thaliana cDNA library. The entire proteins or the Myb domains encoded by the genes were expressed as fusion proteins in Escherichia coli. The DNA-binding domain of the murine c-Myb was also expressed in the same way for use in comparative studies. The fusion proteins were examined for their DNA-binding activity using the animal c-Myb DNA-binding site (MBS) and the binding site of the maize P gene product (PBS). The Myb domain of Atmyb6 bound to PBS more efficiently than to MBS. Complete Atmyb6 and Atmyb7 proteins preferentially bound to PBS but not MBS. This suggests that the in vitro binding consensus sequences for both Atmyb6 and Atmyb7 are similar to PBS. The binding of the Myb domain of Atmyb6 to both PBS and MBS raises the possibility that the protein recognizes multiple sequences in vivo. The third alpha-helix and three adjacent amino acids in the third repeat (R3) of c-Myb was replaced with the analogous sequence of Atmyb6 to create a chimeric Myb protein. This chimeric protein bound to PBS with a low affinity but failed to bind to MBS. Thus the binding pattern of the chimeric Myb protein is similar to that of the Atmyb6. This result suggests ...Continue Reading

Citations

Feb 1, 1997·Trends in Genetics : TIG·C Martin, J Paz-Ares
Apr 5, 2001·Trends in Plant Science·M R HemmC Chapple
Oct 13, 2001·Current Opinion in Plant Biology·R StrackeB Weisshaar
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·I RomeroJ Paz-Ares
Dec 5, 1998·The Plant Journal : for Cell and Molecular Biology·H D KranzB Weisshaar
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·X DanielD Roby
Nov 10, 2011·Biochimica Et Biophysica Acta·Michael B Prouse, Malcolm M Campbell
Jul 10, 2003·The Plant Journal : for Cell and Molecular Biology·Trudi HigginsonRoger W Parish
Mar 14, 2012·The New Phytologist·Hui-Run HuangXue-Jun Ge
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Zhenjun LiQuanhong Yao

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