PMID: 2355730Apr 1, 1990Paper

Isolation of untransformed bovine estrogen receptor without molybdate stabilization

Journal of Steroid Biochemistry
T RatajczakR Hähnel

Abstract

A low concentration estrogen-derivatized affinity resin has been used in a rapid, single step purification of the untransformed estrogen receptor from calf uterine cytosols prepared without sodium molybdata. The procedure isolates the Mr 65,000 estrogen receptor in association with the bovine heat shock protein hsp90. Small amounts of proteolyzed receptor ranging in size from Mr 50,000 to 60,000 are also present in the purified extracts. Results from affinity chromatography of receptor cytosols either untreated or presaturated with estradiol suggest that two proteins of Mr 22,000 and 38,000 are co-purified with the untransformed receptor complex and may represent additional nonhormone-binding components of the native receptor form. Some indication of the stability of protein-protein interactions within the oligomeric complex has been derived from differential salt elution studies with heparin-sepharose and affinity gel-immobilized untransformed receptor. On size exclusion high performance liquid chromatography the untransformed complex eluted with a Stokes radius of 75 +/- 2 A (n = 18), but was shown to be sensitive to extended ultracentrifugal analysis dissociating to the receptor homodimer, sedimentation coefficient 5.3 +/- 0...Continue Reading

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Citations

Dec 15, 2010·Cell Stress & Chaperones·Rudi Kenneth Allan, Thomas Ratajczak
Sep 1, 1990·Steroids·H C AtkinsonR Hähnel
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Segnitz, U Gehring
Jun 8, 2006·Annual Review of Biochemistry·Laurence H Pearl, Chrisostomos Prodromou
Oct 23, 2004·Cell Stress & Chaperones·Amerigo CarrelloThomas Ratajczak
Mar 15, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel L RiggsDavid F Smith
Aug 26, 1996·FEBS Letters·X Zhang, G L Verdine
Aug 19, 2008·FEBS Letters·Tony C LuuWilliam K Chan
May 13, 2009·The International Journal of Biochemistry & Cell Biology·Thomas RatajczakRudi K Allan
Dec 3, 2014·Biochimica Et Biophysica Acta·Steven D Hanes
Mar 29, 2000·Seminars in Cell & Developmental Biology·D F Smith
Feb 6, 2019·Biomolecules·Nadia R ZgajnarMario D Galigniana
Mar 1, 1994·Molecular and Cellular Biology·J L JohnsonD O Toft

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