PMID: 8611655Apr 12, 1996Paper

Isolation of up- and down-regulated cDNAs associated with hepatocellular carcinoma by a subtraction-enhanced display technique

Biochimica Et Biophysica Acta
C G WuR A Chamuleau

Abstract

Identification of gene products exclusively or abundantly expressed in hepatocellular carcinoma (HCC) and in normal liver may yield novel tumor markers. We have isolated 36 up- and down-regulated cDNAs from diethylnitrosamine-induced rat hepatocellular carcinoma and normal liver tissue by using the subtraction-enhanced display technique. Nucleotide sequence analysis revealed that the majority of 20 subtraction-enriched cDNA fragments were well-characterized oncogenes and tumor-associated genes, like c-myc, alpha-prothymosin, p21, glutathione-S transferase (G-ST) and alpha 1-acid glycoprotein (AGP). As demonstrated by Northern blot detection, all of them were preferentially expressed either in HCC or in normal liver (2- to 7-fold). As paradigm, G-ST and AGP were shown to be exclusively overexpressed in tumor nodules by in situ hybridization. In addition, 14 of the remaining 16 novel genes were analysed on Northern blot, 10 of which were differentially expressed in HCC.

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Citations

Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·D C KangP B Fisher
Apr 22, 2008·International Journal of Experimental Pathology·Wilma M FrederiksMarta Cascante
Sep 8, 2004·International Journal of Cancer. Journal International Du Cancer·Xue-Yuan DongWei-Feng Chen
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J S De JongC J Van Noorden
Dec 28, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wilma M FrederiksCornelis J F Van Noorden
Sep 19, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chuan-Ging WuXin Wei Wang
May 16, 1997·International Journal of Cancer. Journal International Du Cancer·C G WuR A Chamuleau

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