Isolation of Vaccine-Like Poliovirus Strains in Sewage Samples From the United Kingdom

The Journal of Infectious Diseases
Manasi MajumdarJavier Martín

Abstract

Environmental surveillance (ES) is a sensitive method for detecting human enterovirus (HEV) circulation, and it is used worldwide to support global polio eradication. We describe a novel ES approach using next-generation sequencing (NGS) to identify HEVs in sewage samples collected in London, United Kingdom, from June 2016 to May 2017. Two different methods were used to process raw sewage specimens: a 2-phase aqueous separation system and size exclusion by filtration and centrifugation. HEVs were isolated using cell cultures and analyzed using NGS. Type 1 and 3 vaccine-like poliovirus (PV) strains were detected in samples collected from September 2016 through January 2017. NGS analysis allowed us to rapidly obtain whole-genome sequences of PV and non-PV HEV strains. As many as 6 virus strains from different HEV serotypes were identified in a single cell culture flask. PV isolates contained only a small number of mutations from vaccine strains commonly seen in early isolates from vaccinees. Our ES setup has high sensitivity for polio and non-PV HEV detection, generating nearly whole-genome sequence information. Such ES systems provide critical information to assist the polio eradication endgame and contribute to the improvement ...Continue Reading

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Citations

Nov 1, 2018·Open Forum Infectious Diseases·Manasi MajumdarJavier Martin
Dec 12, 2018·Microbiology Resource Announcements·T O C FaleyeJ A Adeniji
Aug 13, 2020·Virus Evolution·David JorgensenNicholas C Grassly

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