PMID: 9181123Feb 1, 1997Paper

Isolation of villous microvessels from the human placenta

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
A KacémiJ C Challier

Abstract

A method for isolating the microvessels of the human placental villi has been developed in order to culture perivascular cells. It consists of an initial selection of the villi by serial sieving. The villi retained by the 75 microns sieve were digested by collagenase-dispase. A Percoll gradient permitted the isolation of microvessels still surrounded by stromal fibres and cells. Another digestion by collagenase-dispase eliminated the contaminant elements and allowed, after a new Percoll gradient, microvessels with endothelium, basement membrane and a few perivascular cells to be obtained. Each step of the isolation of microvessels was monitored by light or electron microscopy. Our study confirms the isolation of microvessels embedded in their basement membrane and the preservation of endothelial and perivascular cells after digestion. This method, which has permitted the culture of placental endothelial cells and pericytes, appears of interest for studying microvascular angiogenesis and permeability.

References

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Citations

Nov 23, 2011·The Journal of Endocrinology·Kai Wang, Jing Zheng
Jun 24, 2014·BioMed Research International·Nadia AlfaidySophie Brouillet
Oct 30, 2015·Journal of Translational Medicine·Samuel C WagnerSantosh Kesari
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Antonietta Rosa SiliniOrnella Parolini

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