PMID: 9450238Feb 5, 1998Paper

Isolation of virulent Rhodococcus equi from transtracheal aspirates of foals serodiagnosed by enzyme-linked immunosorbent assay

The Journal of Veterinary Medical Science
T HiguchiShinji Takai

Abstract

Although isolation of Rhodococcus equi from tracheobronchial aspirates is thought to be a definitive diagnosis of R. equi pneumonia in foals, virulence of isolates from the aspirates of infected foals remains obscure. In the present study, transtracheal aspirates were collected from thirty-one 1- to 6-month-old foals, which showed clinical signs of respiratory tract infection, and R. equi isolates were analyzed for the presence of virulence plasmids and virulence-associated antigens. Moreover, this method was compared with a serodiagnosis by an enzyme-linked immunosorbent assay (ELISA) to evaluate the sensitivity of the ELISA. Of the 31 foals, 21 revealed positive cultures for R. equi. Of the 21 foals, 20 (95%) had an ELISA OD value of 0.3 (positive limit of this test) or higher at the initial medical examination. All of the isolates from the aspirates were virulent R. equi, which contained virulence plasmids and expressed virulence-associated antigens. In the remaining 10 foals showing a negative culture for R. equi, 3 foals had positive ELISA titers. Six foals died during the treatment, and necropsy revealed that 5 of the 6 foals had R. equi infection characterized by large abscesses in the lungs, and 3 of the 5 foals also ha...Continue Reading

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Citations

Jul 7, 2011·American Journal of Veterinary Research·M Keith ChaffinLawrence R Bernstein
Apr 3, 2008·Journal of the American Veterinary Medical Association·M Keith ChaffinRonald J Martens
Oct 13, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·T PhumoonnaM W Heuzenroeder
Mar 11, 2003·Clinical and Diagnostic Laboratory Immunology·Steeve GiguèreCorey Miller
Nov 22, 2002·FEMS Immunology and Medical Microbiology·Saíd TaoujiClaire Collobert-Laugier

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