Isolation of WDR and bHLH genes related to flavonoid synthesis in grapevine (Vitis vinifera L.).

Plant Molecular Biology
José Tomás MatusPatricio Arce-Johnson

Abstract

Anthocyanins and tannins are two of the most abundant flavonoids found in grapevine, and their synthesis is derived from the phenylpropanoid pathway. As described for model species such as Arabidopsis thaliana, maize and petunia, the end-point branches of this pathway are tightly regulated by the combinatorial interaction of three families of regulatory factors; MYB, bHLH (also known as MYC) and WDR proteins. Among these, only MYB genes have been previously identified in grapes. Here, we report the isolation of the first members from the WDR and bHLH families found in Vitis vinifera, named WDR1, WDR2 and MYCA1. WDR1 contributed positively to the accumulation of anthocyanins when it was overexpressed in A. thaliana, although it was not possible to determine the function of WDR2 by ectopic expression. The sub-cellular localizations of WDR1 and MYCA1 were observed by means of GFP-fusion proteins, indicating both cytoplasm and nuclear localization, in contrast to the localization of a MYB factor exclusively in the nucleus. The expression patterns of these genes were quantified in coloured reproductive organs throughout development, and correlated with anthocyanin accumulation and the expression profiles of the flavonoid-related MYB...Continue Reading

References

Jul 1, 1991·The Plant Journal : for Cell and Molecular Biology·C MartinE Vrijlandt
May 14, 1999·Trends in Biochemical Sciences·T F SmithE J Neer
Oct 6, 2000·Phytochemistry·J A KennedyA L Waterhouse
May 22, 2002·Journal of Experimental Botany·Rachel GollopAvihai Perl
Oct 9, 2002·Plant Molecular Biology·Kanokporn SompornpailinKazuki Saito
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Antoine BaudryLoïc Lepiniec
Feb 15, 2005·Trends in Plant Science·Nicola A Ramsay, Beverley J Glover
May 11, 2005·Trends in Plant Science·Ronald KoesFrancesca Quattrocchio
Dec 1, 2006·Functional & Integrative Genomics·Grant R CramerJohn C Cushman
Dec 22, 2006·The Plant Journal : for Cell and Molecular Biology·Richard V EspleyAndrew C Allan
Feb 24, 2007·The Plant Journal : for Cell and Molecular Biology·Amanda R WalkerSimon P Robinson
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 28, 2007·Nature·Olivier JaillonUNKNOWN French-Italian Public Consortium for Grapevine Genome Characterization
Feb 19, 2008·Trends in Plant Science·Andrew C AllanWilliam A Laing
Feb 5, 2009·The New Phytologist·Daryl D RowanAndrew C Allan

❮ Previous
Next ❯

Citations

Feb 5, 2011·Plant Cell Reports·Felipe AqueaPatricio Arce-Johnson
Jul 23, 2013·Trends in Plant Science·Laura Jaakola
Feb 1, 2011·Journal of Experimental Botany·Imène HichriVirginie Lauvergeat
Oct 8, 2013·Journal of Experimental Botany·Raja S PayyavulaDuroy A Navarre
Jan 15, 2013·Journal of Experimental Botany·Philippe NicolasFatma Lecourieux
Jan 11, 2014·BMC Plant Biology·Divykriti ChopraMartin Hülskamp
Oct 12, 2013·Biochimica Et Biophysica Acta·Barunava PatraLing Yuan
Nov 29, 2013·Journal of Experimental Botany·Nathalie KuhnSerge Delrot
May 16, 2014·Planta·Md Abdur RahimLivio Trainotti
Apr 23, 2013·Plant Science : an International Journal of Experimental Plant Biology·Yung-Fen HuangPatrice This
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Katia Petroni, Chiara Tonelli
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Antje FellerErich Grotewold
Dec 7, 2013·Journal of Integrative Plant Biology·Wang LiQing Yang
Apr 18, 2014·Plant, Cell & Environment·Linlin LiuBrian Jordan
Jan 29, 2013·Critical Reviews in Biochemistry and Molecular Biology·Takayuki TohgeAlisdair R Fernie
Jun 14, 2015·Journal of Experimental Botany·Laura CostantiniMaria Stella Grando
Apr 9, 2013·Food Chemistry·Xiao-Ling LiYu-Jin Hao
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Véronique CheynierStefan Martens
May 21, 2013·Plant Physiology and Biochemistry : PPB·Grégory CarrierPatrice This
Nov 9, 2014·Molecular Biotechnology·Gabriella De LorenzisAttilio Scienza
Oct 28, 2014·Frontiers in Plant Science·Laura ZorattiLaura Jaakola
Jul 5, 2016·Frontiers in Plant Science·Sangam L DwivediRodomiro Ortiz
May 27, 2014·Journal of Agricultural and Food Chemistry·Jenny Andrea-SilvaFernando M Nunes
Nov 1, 2016·Molecular Breeding : New Strategies in Plant Improvement·Virginia W GitongaFrans A Krens
Jan 20, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min HeXuehao Chen
Dec 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yongzhou LiGuoliang Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.