PMID: 11931267Apr 5, 2002Paper

Isolation of west nile and sindbis viruses from mosquitoes collected in the Nile Valley of Egypt during an outbreak of Rift Valley fever

Journal of Medical Entomology
Michael J TurellSteven M Presley

Abstract

As part of an evaluation of potential vectors of arboviruses during a Rift Valley fever (RVF) outbreak in the Nile Valley of Egypt in August 1993, we collected mosquitoes in villages with known RVF viral activity. Mosquitoes were sorted to species, pooled, and processed for virus isolation both by intracerebral inoculation into suckling mice and by inoculation into cell culture. A total of 33 virus isolates was made from 36,024 mosquitoes. Viruses were initially identified by indirect fluorescent antibody testing and consisted of 30 flaviviruses (all members of the Japanese encephalitis complex, most probably West Nile [WN] virus) and three alphaviruses (all members of western equine encephalitis complex, most probably Sindbis). The identity of selected viruses was confirmed by reverse transcriptase-polymerase chain reaction and sequencing. Culex antennatus (Becker) and Culex perexiguus Theobald accounted for five (17%) and 23 (77%) of the WN virus isolations, respectively. Despite isolation of viruses from 32 pools of mosquitoes (both WN and Sindbis viruses were isolated from a single pool), RVF virus was not isolated from these mosquitoes, even though most of them are known competent vectors collected during an ongoing RVF ou...Continue Reading

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Citations

Oct 2, 2008·Journal of Medical Entomology·L OrshanH Pener
Dec 30, 2011·PLoS Neglected Tropical Diseases·Veronique ChevalierRené Rakotondravao
Jan 14, 2017·PLoS Neglected Tropical Diseases·Mélissanne de WispelaereValérie Choumet
Sep 13, 2007·Journal of the American Mosquito Control Association·N HaddadH Bouharoun-Tayoun
Jan 30, 2019·PLoS Neglected Tropical Diseases·Sana EybpooshMostafa Salehi-Vaziri
Oct 26, 2013·International Journal of Environmental Research and Public Health·Olivier EnglerNicholas Johnson
May 6, 2014·Journal of Materials Science. Materials in Medicine·Iu BykovaI Khlusov
Jul 27, 2017·PloS One·Dhan Kumar PantPadam Raj Bist
Jul 17, 2015·Journal of the American Mosquito Control Association·Abdelbaset B ZayedKenneth J Linthicum
Feb 14, 2017·Transboundary and Emerging Diseases·A J GolnarG L Hamer
Aug 14, 2020·Parasites & Vectors·Gail E ChapmanMatthew Baylis

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