PMID: 6163467Feb 13, 1981Paper

Isolation, properties, immunological specificity and localization of mouse testicular hyaluronidase

Biochimica Et Biophysica Acta
G S Gupta, E Goldberg

Abstract

Hyaluronidase (hyaluronate 4-glycanohydrolase, EC 3.2.1.35) was purified from mouse testes by ion-exchange chromatography, Sephadex G-200 filtration and Con A-agarose affinity chromatography. The final preparation had 94-fold purity and 12.2 units spec. act. of the enzyme (unit of specific activity = mumol N-acetylglucosamine released/h per mg protein at 37 degrees C and pH 4.5). Hyaluronidase is relatively heat stable and loses 10-20% of its activity at 50-55 degrees C for 10 min. Ea for eat denaturation of enzyme is 42-45 kcal between 45 an 63 degrees C. The Michaelis constant of mouse testicular hyaluronidase is 1.1 mg/ml hyaluronic acid. Antibodies to the purified enzyme were produced in rabbits and showed a single precipitin line by Ouchterlony gel diffusion. Antiserum to hyaluronidase inhibited enzyme activity by 25%. Immunologically, mouse testicular hyaluronidase is species specific. Tissue extracts of mouse vital organs, except testes and epididymis did not react with the antisera, though nonspecific precipitation occurred between intestinal extracts and anti-hyaluronidase serum. Hyaluronidase was localized in testis sections by indirect immunofluorescence. A specific dark green fluorescence was localized on cell bound...Continue Reading

References

Oct 13, 1978·Molecular and Cellular Biochemistry·J T Dulaney
Jun 1, 1977·Developmental Biology·M Hintz, E Goldberg
Jun 24, 1975·Biochimica Et Biophysica Acta·C H Yang, P N Srivastava
Jan 1, 1974·Annual Review of Biochemistry·R A McRorie, W L Williams
Jun 1, 1972·Biology of Reproduction·C B Metz
Apr 1, 1973·FEBS Letters·A Y KhorlinA N Milishnikov
Jul 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·C B MetzA E Castro
Oct 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·A C Menge
Apr 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·A C Menge
Jan 1, 1969·Journal of Chemical Education·J M Brewer, R B Ashworth
Jan 1, 1959·Acta Anatomica·J A YAEGER, T O ANDERSON
Dec 1, 1964·Journal of Reproduction and Fertility·R E MANCINIM NEMIROVSKY
Jan 1, 1947·The Biochemical Journal·G I Swyer

❮ Previous
Next ❯

Citations

Aug 25, 1993·Molecular and Cellular Biochemistry·G S Gupta, R G Kinsky
Jan 1, 1986·Radiation and Environmental Biophysics·P K Sharma, G S Gupta
Jan 1, 1989·Somatic Cell and Molecular Genetics·B Fiszer-Szafarz, E De Maeyer
Jun 1, 1985·Journal of Dental Research·C N Bertolami, D G Ellis
Jan 1, 1989·Archives of Andrology·D K Kapur, G K Ahuja
Nov 14, 1983·Biochimica Et Biophysica Acta·G S Gupta, G P Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.