Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei

Protein Expression and Purification
Priyanka PriyankaBarry J Ryan

Abstract

The Pseudomonas sp. have been long recognized for their exogenous lipolytic activities yet the genus still contains a lot of unexplored strains. Due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions Pseudomonas sp. are of great interest for biotechnological applications. In this study, a new extracellularly produced lipolytic enzyme from Pseudomonas sp. (P. reinekei) was purified and characterized. The production of lipase from P. reinekei (H1) was enhanced 10-fold by optimizing the nitrogen source. The 50 kDa H1 lipase was purified using negative and positive mode anion exchange chromatography. The purified lipase was active over a broad pH range (5.0-9.0) and was stable for 24 h at 40 °C. The lipase showed significant stability, and indeed activation, in the presence of organic solvents with log P ≥ 2.0. These features render this lipase of interest as a biocatalyst for applications such as biodiesel production, detergent formulations and biodegradation of oil in the environment.

Citations

Feb 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Miroslav Pohanka
May 22, 2020·Frontiers in Bioengineering and Biotechnology·Letícia L RadeLeticia M Zanphorlin
Jun 21, 2020·Environmental Science and Pollution Research International·Vinod Kumar Nathan, Mary Esther Rani
Oct 13, 2020·Applied Biochemistry and Biotechnology·Maria de Fátima M de FreitasLuciana R B Gonçalves
Jul 13, 2021·AIMS Microbiology·Divakar Dahiya, Poonam Singh Nigam

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