Isolation, purification and characterization of silk protein sericin from cocoon peduncles of tropical tasar silkworm, Antheraea mylitta

International Journal of Biological Macromolecules
Rupesh DashSubhas C Kundu

Abstract

A high molecular weight water-soluble glue protein, sericin was identified in the cocoon peduncle (a strong thread connecting the cocoons to the branches of the tree with a ring) of the tropical tasar silkworm, Antheraea mylitta. The sericin was isolated by 8M urea containing 1% sodium dodecyl sulfate and beta-mercaptoethenol (2%) or by 1% sodium chloride. The protein was purified by gel filtration chromatography. In SDS-PAGE, a single band of approximately 200kDa was detected both in non-reducing and reducing conditions. Amino acid analysis showed that the protein is enriched in glycine and serine. There is a slight difference observed in amino acid composition between the sericin from cocoon peduncle and cocoon of A. mylitta. Secondary structure estimation by circular dichroism spectrometry showed 36.7% beta-sheets, 52.7% random coils, 10.6% turns and no helices.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
May 5, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A DattaS C Kundu
Aug 3, 2001·Insect Biochemistry and Molecular Biology·A DattaS C Kundu
Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Yoko TakasuKozo Tsubouchi

❮ Previous
Next ❯

Citations

Feb 26, 2010·Pakistan Journal of Biological Sciences : PJBS·P SrihanamY Srisuwan
Jul 16, 2011·Journal of Ayurveda and Integrative Medicine·Mir Mahdi Ali, Sarasa Bharati A Arumugam
Dec 1, 2012·Biotechnology Advances·Ali TamayolDavid Juncker
Nov 3, 2015·Biotechnology Advances·Lallepak LamboniQun Wang
Mar 14, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rupesh DashS C Kundu
Jun 23, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Lindsay S WrayDavid L Kaplan
Oct 3, 2012·International Journal of Biological Macromolecules·Haesung YunKi Hoon Lee
May 18, 2016·Insect Biochemistry and Molecular Biology·Zhaoming DongQingyou Xia
Aug 16, 2016·BioMed Research International·Neety SahuSubhas C Kundu
Jun 23, 2010·Journal of Biomaterials Science. Polymer Edition·Joydip KunduS C Kundu
Jan 14, 2012·Biopolymers·S C KunduAnanta K Ghosh
Sep 1, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sharda GuptaArindam Bit
Feb 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Linhao LiLi Yang
Jun 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Guangqiang FangXin Chen
Apr 12, 2012·Macromolecular Bioscience·Khoon S LimPenny J Martens
Feb 18, 2021·ACS Biomaterials Science & Engineering·Li ZhangWeiliang Shen
May 10, 2011·International Journal of Biological Macromolecules·Biman B MandalS C Kundu
Aug 25, 2021·Biomedical Materials·Deboki NaskarSubhas C Kundu
Dec 24, 2021·Journal of Biomolecular Structure & Dynamics·Anurupa Goswami, Dipali Devi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.