Isolation, purification, and characterization of the major autolysin from Pseudomonas aeruginosa

Canadian Journal of Microbiology
S R Watt, Anthony J Clarke

Abstract

The major (26 kDa) autolysin from Pseudomonas aeruginosa was purified to apparent homogeneity by a combination of preparative electrophoresis, ion-exchange, and dye-ligand chromatographies. This purification was facilitated by the development of a spot-assay that involved the spotting and subsequent incubation of autolysin samples on polyacrylamide gels containing peptidoglycan. The pl of the 26-kDa autolysin was determined to be between 3.5 and 4 and disulfide bonds within the enzyme were essential for activity. The autolysin catalyzed the release of reducing sugars from the peptidoglycans of Pseudomonas aeruginosa and Escherichia coli indicating it to be a beta-glycosidase. It was ineffective at hydrolysing the peptidoglycan from Gram-positive bacteria and the O-acetylated peptidoglycans from either Proteus mirabilis or Staphylococcus aureus. The N-terminal sequence of the purified autolysin was determined to be His-Glu-Pro-Pro-Gly. The 26-kDa autolysin together with a 29-kDa autolysin was determined to be secreted into the medium by a mechanism that involves the production and release of surface membrane vesicles during normal growth, but the enzymes were not found free and active in culture broth supernatants.

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Citations

Feb 1, 2008·The Journal of Biological Chemistry·Edie M Scheurwater, Anthony J Clarke
Mar 23, 2001·Analytical Biochemistry·H Strating, A J Clarke
Sep 1, 2018·Canadian Journal of Microbiology·Anthony J Clarke
Nov 29, 2002·Canadian Journal of Microbiology·Kelly L MacDonald, Terry J Beveridge

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