Isolation, purification, LC-MS/MS characterization and reactive oxygen species induced by fumonisin B1 in VERO cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Giuseppe MecaJ Mañes

Abstract

Fumonisins are mycotoxins produced by Fusarium verticillioides that commonly contaminate maize and maize products. The present work shows the results of a comparative study of three different fermentation's techniques (solid and liquid medium of corn and a solid agarized medium) for the production of fumonisins B(1), B(2) and B(3) with strains of F. verticillioides. The solid medium of corn was the most effective in the production of fumonisins, being Fumonisin B(1) the one produced with higher concentration, so the extract obtained by solid fermentation process was used for FB(1) purification. Fumonisins characterization and quantification were performed with reversed-phase high-performance liquid chromatography with electrospray ionization triple quadrupole tandem mass spectrometry. The role of production of reactive oxygen species (ROS) in Fumonisin B(1) mediated toxicology has not been fully addresses in studies exploring FB(1) toxicity. It is evaluated the level of ROS production in kidney cell line (VERO) exposed to 1, 5 and 10 μM of FB(1) for 0.5-100 min. The ROS level was detected using a fluorescence probe, 2',7'-dichlorofluorescein diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein...Continue Reading

References

May 4, 2000·Journal of Agricultural and Food Chemistry·S M Poling, R D Plattner
May 22, 2001·Environmental Health Perspectives·K A VossJ K Porter
May 22, 2001·Environmental Health Perspectives·R T RileyA H Merrill
Dec 4, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F GalvanoM Renis
Sep 1, 2004·Toxicology·Helene Stockmann-JuvalaKai Savolainen
Dec 29, 2004·Free Radical Research·Helene Stockmann-JuvalaKai Savolainen
Feb 27, 2007·The Science of the Total Environment·Maria de la Luz Z BasilicoJuan Carlos Basilico
Feb 27, 2009·Human & Experimental Toxicology·H Stockmann-Juvala, K Savolainen
Mar 11, 2009·Human & Experimental Toxicology·Am DomijanM Kalafatic
Jun 3, 2009·Toxicon : Official Journal of the International Society on Toxinology·Xiaoou ZhangLaifu Zhong
Jul 15, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E FerrerM J Ruiz
Aug 5, 2009·Molecular Nutrition & Food Research·Rita DornetshuberWalter Berger

❮ Previous
Next ❯

Citations

Feb 20, 2014·Archives of Environmental Contamination and Toxicology·Fiorenza MinerviniAngelo Visconti
May 10, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A GarbettaF Minervini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.