Abstract
In this study, Ligustrum lucidum flowers as raw material, the extraction, isolation and coagulatory activity of polysaccharides were carried out for the first time. The crude polysaccharide was obtained by hot water extraction and ethanol precipitation, and preliminarily purified by Sevage method and D101 macroporous resin. Then the polysaccharide was further purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, respectively. The structural characteristics were detected by LC, GC, FT-IR and NMR. Furthermore, the coagulatory activity of the polysaccharides were investigated by APTT, TT, PT and FIB assays in vitro. The results demonstrated that four polysaccharides were isolated from flowers of L. lucidum, named as LLP-1a, LLP-1b, LLP-2 and LLP-3, and the yields were 0.039, 0.0054, 0.0055 and 0.017%, respectively based on the weight of the dried flowers. The four polysaccharides components were free of nucleic acids and proteins, and their average molecular weights were 25,912, 64,919, 3,940,246 and 2,975,091 g/mol, respectively. The monosaccharide compositions of LLp-1a were L-rhamnose, L-arabinose, D-xylose, D-glucose and D-galactose (molar ratio of 3.16: 2.46: 1.00: 7.27: 4.22). Only D-galactose was detected ...Continue Reading
References
Nov 8, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xingbin YangQibing Mei
Nov 25, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kung-chi ChanWan-ju Chao
Sep 16, 2009·Free Radical Biology & Medicine·Jane A Leopold, Joseph Loscalzo
May 17, 2011·Indian Journal of Critical Care Medicine : Peer-reviewed, Official Publication of Indian Society of Critical Care Medicine·Pranav Sikka, V K Bindra
Feb 9, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aida Firdaus Muhammad Nurul AzmiYazid Abdul Manap
Apr 10, 2012·Talanta·Eva Gómez-OrdóñezPilar Rupérez
Aug 31, 2013·Carbohydrate Polymers·Jian-Hua XieMing-Yong Xie
Mar 25, 2014·Journal of Asian Natural Products Research·Li-Jun JianJia-Wei He
Jul 16, 2014·Oncology Reports·Bing HuShuang-Shuang Wang
Feb 16, 2016·Carbohydrate Polymers·Weirong CaiQinbao Fu
May 1, 2016·Glycoconjugate Journal·Yiping JiangWei Xiao
May 27, 2016·Food & Function·Song-Zi XieJian-Ping Luo
Jul 31, 2016·Carbohydrate Polymers·Shudong HeYongkang Ye
Jul 31, 2016·Carbohydrate Polymers·Wei TangMingyong Xie
Dec 19, 2016·Carbohydrate Polymers·Jing FanMengdi Sun
Feb 6, 2017·International Journal of Biological Macromolecules·Shuang WangWeimin Wang
Mar 2, 2017·Chemistry Central Journal·Jinmei WangWen-Yi Kang
Apr 24, 2017·Carbohydrate Polymers·Nadezhda E UstyuzhaninaAnatolii I Usov
May 10, 2017·Food & Function·Pingyao XieWenyi Kang
Citations
Nov 14, 2019·Journal of Food Biochemistry·Li-Li GongYan Chen
Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan RenAntonio Del Rio Flores
Dec 7, 2018·Chemistry Central Journal·Zhenhua YinWenyi Kang
Jan 11, 2020·Scientific Reports·Shun Mei LinSangyong Lim
Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenhua YinWenyi Kang
Feb 26, 2021·International Journal of Biological Macromolecules·Selina Mawunyo Ayivi-TosuhGuiyun Wang
Jan 19, 2021·ACS Omega·Wanchen ZhaiHongli Zhou
Dec 1, 2021·Critical Reviews in Food Science and Nutrition·Yinxin CuiJike Lu