Isolation, purification, structural analysis and coagulatory activity of water-soluble polysaccharides from Ligustrum lucidum Ait flowers

Chemistry Central Journal
Zhenhua YinWenyi Kang

Abstract

In this study, Ligustrum lucidum flowers as raw material, the extraction, isolation and coagulatory activity of polysaccharides were carried out for the first time. The crude polysaccharide was obtained by hot water extraction and ethanol precipitation, and preliminarily purified by Sevage method and D101 macroporous resin. Then the polysaccharide was further purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, respectively. The structural characteristics were detected by LC, GC, FT-IR and NMR. Furthermore, the coagulatory activity of the polysaccharides were investigated by APTT, TT, PT and FIB assays in vitro. The results demonstrated that four polysaccharides were isolated from flowers of L. lucidum, named as LLP-1a, LLP-1b, LLP-2 and LLP-3, and the yields were 0.039, 0.0054, 0.0055 and 0.017%, respectively based on the weight of the dried flowers. The four polysaccharides components were free of nucleic acids and proteins, and their average molecular weights were 25,912, 64,919, 3,940,246 and 2,975,091 g/mol, respectively. The monosaccharide compositions of LLp-1a were L-rhamnose, L-arabinose, D-xylose, D-glucose and D-galactose (molar ratio of 3.16: 2.46: 1.00: 7.27: 4.22). Only D-galactose was detected ...Continue Reading

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Citations

Aug 31, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan RenAntonio Del Rio Flores
Dec 7, 2018·Chemistry Central Journal·Zhenhua YinWenyi Kang
Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenhua YinWenyi Kang
Feb 26, 2021·International Journal of Biological Macromolecules·Selina Mawunyo Ayivi-TosuhGuiyun Wang
Dec 1, 2021·Critical Reviews in Food Science and Nutrition·Yinxin CuiJike Lu

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Methods Mentioned

BETA
infrared spectroscopy
NMR
nuclear magnetic resonance

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