PMID: 11607217Oct 1, 1991Paper

Isolation, sequence analysis, and physiological properties of enkephalins in the nervous tissue of the shore crab Carcinus maenas L

Proceedings of the National Academy of Sciences of the United States of America
W LüschenP P Jaros

Abstract

[Leu]- and [Met]enkephalin from thoracic ganglia of the shore crab Carcinus maenas have been purified to homogeneity by a reversed-phase HPLC procedure. Automated gas-phase sequencing revealed a primary structure identical with that of enkephalins from vertebrates. The immunoreactive material in different parts of the nervous system of C. maenas was measured by RIA. Isolated crustacean [Leu]enkephalin shows physiological properties identical to synthetic [Leu]enkephalin in our bioassay. The enkephalinergic effect has been monitored by two bioassays: (i) inhibition of release of crustacean hyperglycemic hormone from isolated eyestalks of C. maenas, and (ii) decreased effect on blood glucose level in the fiddler crab Uca pugilator. The decrease in blood sugar level induced by [Leu]enkephalin was antagonized by naloxone, indicating a receptor-mediated process. In sequence analysis, the thoracic ganglion yielded 3 pmol of [Leu]enkephalin and approximately 1 pmol of [Met]enkephalin. The presence of enkephalins in crustaceans shows an earlier phylogenetic origin of opioids than presumed.

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Citations

Jul 1, 1993·Cell and Tissue Research·D R Nässel
Mar 1, 1995·Pharmacology, Biochemistry, and Behavior·A M Godoy, H Maldonado
Nov 1, 1992·Peptides·G A OlsonA J Kastin
Jun 5, 2003·Brain Research. Molecular Brain Research·V Gonzalez NuñezR E Rodríguez
Feb 5, 1998·Brain Research. Molecular Brain Research·M Salzet, G Stefano
Jan 1, 1996·Brain Research Bulletin·A DelorenziH Maldonado
Oct 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·R SarojiniM Fingerman
Feb 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·C Y LinC M Kuo
Jun 21, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Engin BojnikSandor Benyhe
Aug 21, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew E ChristiePatsy S Dickinson
Jan 21, 2003·Mass Spectrometry Reviews·Robert M DoresPhillip B Danielson
Dec 8, 2011·General and Comparative Endocrinology·Simon George WebsterHeinrich Dircksen
Feb 18, 2011·Behavioural Processes·Stuart Barr, Robert W Elwood
Jan 15, 2008·Seminars in Cancer Biology·George B Stefano, Richard Kream
Feb 4, 1994·Brain Research·J AmmermüllerU Janssen-Bienhold
Nov 18, 2005·General and Comparative Endocrinology·Akiyoshi Takahashi, Hiroshi Kawauchi
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Jul 4, 2003·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Hong-Shin ZouChi-Ying Lee
Jul 4, 2015·The Journal of Experimental Biology·Pascal FossatJean-Paul Delbecque

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