PMID: 9531631Jun 6, 1998Paper

Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the brnQ gene product

Archives of Microbiology
Andreas TauchJ Kalinowski

Abstract

By complementation analysis of an isoleucine-uptake-deficient Escherichia coli strain, it was shown that a 1.6-kb HindIII-StuI fragment of Corynebacterium glutamicum ATCC 13032, located downstream of the aecD gene, encodes an isoleucine uptake system. Sequence analysis revealed that the complementing fragment carried an open reading frame, termed brnQ, that encodes a protein with sequence similarities to branched-chain amino acid carriers of gram-positive and gram-negative bacteria. The brnQ gene specifies a predominantly hydrophobic protein of 426 amino acid residues with a calculated molecular mass of 44.9 kDa. A topology prediction by neural network computer analysis suggests the existence of 12 hydrophobic segments that most probably form transmembrane alpha-helices. A C. glutamicum mutant strain harboring a defined deletion of brnQ in the chromosome showed a considerably lower isoleucine uptake rate of 0.04 nmol min-1 mg (dry mass)-1 as compared to the wild-type strain rate of 1.2 nmol min-1 mg (dry mass)-1. Overexpression of brnQ by means of a tac promotor resulted in an elevated uptake rate for isoleucine of 11.3 nmol min-1 mg (dry mass)-1. Evidently, the brnQ gene encodes the only transport system in C. glutamicum direc...Continue Reading

Citations

Mar 17, 2007·Applied Microbiology and Biotechnology·Georg Sindelar, Volker F Wendisch
Oct 22, 2009·Applied Microbiology and Biotechnology·Jin Hwan Park, Sang Yup Lee
Jun 27, 2012·Journal of Industrial Microbiology & Biotechnology·Xixian XieNing Chen
Sep 2, 2003·Journal of Biotechnology·Oliver Kirchner, Andreas Tauch
Jan 9, 2004·Applied and Environmental Microbiology·Balasubramanian Ganesan, Bart C Weimer
Mar 25, 2008·RNA·Alexey G VitreschakMikhail S Gelfand
May 13, 2014·Applied Microbiology and Biotechnology·Marco OldigesBastian Blombach
Jul 22, 2014·Proteins·Ake VastermarkMilton H Saier
Jun 3, 2010·Biotechnology Journal·Jin Hwan Park, Sang Yup Lee
Dec 31, 2014·Infection and Immunity·Julienne C KaiserDavid E Heinrichs
Aug 16, 2015·Applied and Environmental Microbiology·Andrea MichelMichael Bott
May 6, 2019·Critical Reviews in Biotechnology·Ying-Yu WangWei-Guo Zhang
Jun 1, 2020·Biotechnology and Applied Biochemistry·Yanchao ZhangXiaoyuan Wang
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang

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