Isoliquiritigenin Induces Mitochondrial Dysfunction and Apoptosis by Inhibiting mitoNEET in a Reactive Oxygen Species-Dependent Manner in A375 Human Melanoma Cells

Oxidative Medicine and Cellular Longevity
Xiao-Yu ChenQiu-Sheng Zheng

Abstract

The mitochondrial protein mitoNEET is a type of iron-sulfur protein localized to the outer membrane of mitochondria and is involved in a variety of human pathologies including cystic fibrosis, diabetes, muscle atrophy, and neurodegeneration. In the current study, we found that isoliquiritigenin (ISL), one of the components of the root of Glycyrrhiza glabra L., could decrease the expression of mitoNEET in A375 melanoma cells. We also demonstrated that mitoNEET could regulate the content of reactive oxygen species (ROS), by showing that the ISL-mediated increase in the cellular ROS content could be mitigated by the mitoNEET overexpression. We also confirmed the important role of ROS in ISL-treated A375 cells. The increased apoptosis rate and the decreased mitochondrial membrane potential were mitigated by the overexpression of mitoNEET in A375 cells. These findings indicated that ISL could decrease the expression of mitoNEET, which regulated ROS content and subsequently induced mitochondrial dysfunction and apoptosis in A375 cells. Our findings also highlight mitoNEET as a promising mitochondrial target for cancer therapy.

References

Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J A SimoneauD E Kelley
Apr 12, 2002·Journal of Neurochemistry·Yuanbin LiuDavid Schubert
Jul 4, 2003·The Journal of Biological Chemistry·Qun ChenEdward J Lesnefsky
Oct 17, 2003·The Journal of Physiology·Julio F Turrens
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Mar 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sandra E WileyJack E Dixon
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Michal SchwarzAtan Gross
Dec 1, 2007·Biochemical and Biophysical Research Communications·Guillermo L TaminelliTomás A Santa-Coloma
Dec 27, 2008·Free Radical Biology & Medicine·Defang LiJi Li
Aug 8, 2009·Annual Review of Genetics·Chunxin Wang, Richard J Youle
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
Aug 18, 2010·Biochimica Et Biophysica Acta·David L Vaux
Oct 22, 2010·Nature·Nick Lane, William Martin
Dec 4, 2010·International Journal of Cancer. Journal International Du Cancer·Chaoyang LiuWenhua Li
Jan 11, 2011·Trends in Pharmacological Sciences·Barry Halliwell
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·John A ZurisRachel Nechushtai
Jun 15, 2012·Journal of Asian Natural Products Research·Xuan YuanZhi-Ping Wang
Jan 11, 2013·Oxidative Medicine and Cellular Longevity·Xiaoyu ChenQiusheng Zheng
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yang-Sung SohnRon Mittler
Dec 21, 2013·Journal of Cell Science·Nataly Shulga, John G Pastorino
Oct 4, 2014·Free Radical Biology & Medicine·Christine C Winterbourn
Nov 2, 2014·Cell Death and Differentiation·G JacqueminD Martinvalet
Feb 13, 2015·Future Oncology·Peter GorayskiMatthew Foote
Apr 25, 2015·Phytotherapy Research : PTR·Fu PengJianping Chen
May 23, 2015·Biochemical and Biophysical Research Communications·Sten OrreniusBoris Zhivotovsky
Apr 14, 2016·Seminars in Nuclear Medicine·Abdullah Norain, Ekaterina Dadachova

❮ Previous
Next ❯

Citations

Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Colin H LipperPatricia A Jennings
Jan 7, 2021·Cancers·Kai-Lee WangShih-Min Hsia
Aug 12, 2021·Journal of Clinical Biochemistry and Nutrition·Keiichi HiramotoShosuke Kawanishi

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assay
ELISA
Protein Assay

Software Mentioned

SPSS
CellQuest

Related Concepts

Related Feeds

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.